Biotin kits, HOOK™
About this item
HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.
- Optimizer Buffer™ for improved coupling efficiency
- SpinOUT™ gel filtration columns for rapid (<10 minutes) purification
- Biotin assay reagents to determine level of biotin incorporation
- Labels 1 to 10 mg protein/reaction
- Suitable for 10 coupling reactions
The kits include EDC as the carbodiimide crosslinker in the carboxyl coupling kits and sodium meta-periodate for carbohydrate oxidation in the carbohydrate coupling kits. Kit also contains a specific Optimizer Buffer™ that provides the optimal reaction conditions for each HOOK™ Biotin Reagent.
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Product Details & Documents
HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.
- Optimizer Buffer™ for improved coupling efficiency
- SpinOUT™ gel filtration columns for rapid (<10 minutes) purification
- Biotin assay reagents to determine level of biotin incorporation
- Labels 1 to 10 mg protein/reaction
- Suitable for 10 coupling reactions
The kits include EDC as the carbodiimide crosslinker in the carboxyl coupling kits and sodium meta-periodate for carbohydrate oxidation in the carbohydrate coupling kits. Kit also contains a specific Optimizer Buffer™ that provides the optimal reaction conditions for each HOOK™ Biotin Reagent.
Documents
Product Family Options
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- BS-01NHS-Biotin kit
- BS-02NHS-LC-Biotin kit
- BS-03NHS-LC-LC-Biotin kit
- BS-04NHS-SS-Biotin kit
- BS-05NHS-dPEG₄™-Biotin kit
- BS-06Sulpho-NHS-Biotin kit
- BS-07Sulpho-NHS-LC-Biotin kit
- BS-08Sulpho-NHS-LC-LC-Biotin kit
- BS-09Sulpho-NHS-SS-Biotin kit
- BS-10PFP-Biotin kit
- BS-11PEG₂-Iodoacetyl-Biotin kit
- BS-12Iodoacetyl-LC-Biotin kit
- BS-13Biotin-PDA kit
- BS-14Biotin-BMCC kit
- BS-16Biotin-PEG₂-Amine kit
- BS-17Biotin-PEG₃-LC-Amine kit
- BS-18Biotin-Hydrazide kit
- BS-19Biotin-LC-Hydrazide kit
Accessories
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- Optimizer Buffer™-V, 5X
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- Optimizer Buffer™-III, 5X
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- Optimizer Buffer™-IV, 5X
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- Optimizer Buffer™-VI, 5X
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- Optimizer Buffer™-II,5X
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- Optimizer Buffer™-I, 5X