Specifications

Particle Size:
90 µm
Pk:
200 mL
Description:
Phenyl Sepharose™ CL-4B
Environmentally Preferable:
pH range:
3 - 12
Matrix:
Cross-linked agarose, 4%, spherical
Ligand:
Phenyl
Storage temperature:
4 - 30 °C
Binding capacity:
HSA (Mr 68 000) 15-20 mg/ml drained resin, ß-lactoglobulin (Mr 18 000) 3-5 mg/ml drained resin
Ligand density:
40 μmole/ml drained medium
Flow rate:
150 cm/h
Type:
Liquid

Specifications

About this item

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

Phenyl immobilized on 4% cross-linked agarose
Aromatic ligand
Stable in all commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride
Negligible volume variation due to changes in pH or ionic strength

Octyl Sepharose® CL-4B and Phenyl Sepharose® CL-4B are covalently coupled to a cross-linked 4% agarose matrix by ether linkage, resulting in hydrophobic gels with minimal leakage and no ionic properties.