Affinity chromatography columns, GSTrap™ FF
About this item
GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
- Fast and simple one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Easy scale-up by connecting the columns in series
- Sepharose 4 Fast Flow provides good flow properties
GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione.
The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
4 Options Available Below
Product Details & Documents
GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
- Fast and simple one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Easy scale-up by connecting the columns in series
- Sepharose 4 Fast Flow provides good flow properties
GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione.
The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
Documents
Cytiva CyDye Licensing Statements Cytiva General Licensing Statements
Additional Product Details
Specifications for Product Family
Specifications
- Catalog No:
- 17-5130-02
- 17-5130-01
- 17-5131-01
- 17-5131-02
- Max. pressure:
- 5 bar
- 5 bar
- 5 bar
- 5 bar
- pH range:
- 3 - 12
- 3 - 12
- 3 - 12
- 3 - 12
- Storage:
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
Specifications
Product Family Options
Product Information
- Column hardwareParticle SizeBed dimensionsCapacityFlow rateFlow velocityAvailabilityPrice
- 17-5130-02GSTrap™ FFSpecifications:
More
Specifications
Cat. No.17-5130-02Max. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 17-5130-01GSTrap™ FFSpecifications:
More
Specifications
Cat. No.17-5130-01Max. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 17-5131-01GSTrap™ FFSpecifications:
More
Specifications
Cat. No.17-5131-01Max. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 17-5131-02GSTrap™ FFSpecifications:
More
Specifications
Cat. No.17-5131-02Max. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol