You searched for: Western Blotting Reagents

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

Supplier: G-Biosciences

Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.

Supplier: G-Biosciences

These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.

Supplier: VWR Chemicals

VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.

Supplier: Merck

The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

Supplier: Thermo Fisher Scientific

The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Supplier: G-Biosciences

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

Supplier: G-Biosciences

Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Supplier: G-Biosciences

G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.

Supplier: G-Biosciences

These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.

Supplier: Cytiva

Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

Supplier: Rockland Immunochemicals

NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

Supplier: G-Biosciences

G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

Supplier: Biotium

NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Supplier: Thermo Fisher Scientific

Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.

Supplier: Thermo Fisher Scientific

Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Supplier: Thermo Fisher Scientific

Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

Supplier: Thermo Fisher Scientific

NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.

Supplier: Thermo Fisher Scientific

SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.

Supplier: Thermo Fisher Scientific

The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

Supplier: Thermo Fisher Scientific

Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

Supplier: Thermo Fisher Scientific

The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

Supplier: Thermo Fisher Scientific

SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.