Agarose and Acrylamide
Agarose and acrylamide are the most commonly used electrophoresis gels for their versatility with buffers and ability to generate reproducible results. Agarose typically runs horizontal tests to resolve large DNA fragments while acrylamide runs vertical separations for shorter nucleic acids. Easy to handle, the gels are chosen based on the high resolution desired and the samples. The concentration or ratio of the agarose and acrylamide will determine the overall working porosity.
Agarose, SeaKem® LE
Supplier: LONZA
SeaKem® LE is a high gel strength, low EEO, standard gelling temperature agarose perfect for electrophoresis and other molecular biology applications.
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Agarose I, Pierce™
Supplier: Thermo Fisher Scientific
Agarose I (molecular biology grade) is a highly purified and carefully blended formulation that provides uniform lot-to-lot consistency for preparation of electrophoresis gels.
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UltraSpec-Agarose™, DNA electrophoresis grade
Supplier: EDVOTEK
Electrophoresis Gels, UltraSpec-Agarose™
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Agarose, SeaKem® Genetic Technology Grade™ (GTG)
Supplier: LONZA
SeaKem® is a high gel strength, low EEO, standard gelling temperature agarose perfect for electrophoresis and other molecular biology applications.
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Agarose, SeaKem® HE
Supplier: LONZA
SeaKem® HE agarose is a high EEO agarose that provides enhanced resolution in immunoelectrophoresis (IEP), crossed-IEP, counterimmunoelectrophoresis (CIEP), and serum protein electrophoresis.
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Agarose, SeaKem® HGT
Supplier: LONZA
SeaKem® HGT agarose is a high-gelling temperature (42 °C) agarose that exhibits high gel strength and clarity. It is primarily used in protein electrophoresis applications such as counterimmunoelectrophoresis (CIEP) and crossed immunoelectrophoresis.
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Agarose, NuSieve® Genetic Technology Grade™ (GTG)
Supplier: LONZA
NuSieve® Genetic Technology Grade™ (GTG) Agarose is a low melting temperature (65 °C) agarose that finely resolves DNA fragments, PCR and RT-PCR products ranging from 10 to 1000 bp.
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Agarose, low gelling temperature
Supplier: Thermo Fisher Scientific
Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.
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Agarose, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
This agarose is a granular, free-flowing powder.
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Acrylamide/ Bis-Acrylamide
Supplier: G-Biosciences
Acrylamide (electrophoresis grade) is supplied as a powder or a 40% solution in ultrapure water. Bisacrylamide (Bis (N,N’-methylenebisacrylamide)) (electrophoresis grade) is supplied as a powder or a 2% solution in ultrapure water.
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Acrylamide/Bis-Acrylamide
Supplier: Thermo Fisher Scientific
Premix of acrylamide with the common cross-linking reagent of final ratio 29:1.
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Acrylamide, electrophoresis grade, MP Biomedicals
Supplier: MP Biomedicals
Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules.
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Agarose, immunodiffusion grade, MP Biomedicals
Supplier: MP Biomedicals
Agarose is the most popular medium for immunoelectrophoresis because of the large pore size for rapid diffusion, and for low background staining by Coomassie Blue G stain. The low EEO and high gel strength is specifically selected and tested for immunoelectrophoresis and immunodiffusion.
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Acrylamide/Bis-Acrylamide, LIQUI-GEL™ solution 40% (19:1), MP Biomedicals
Supplier: MP Biomedicals
Liqui-Gel is a 40% (w/v) solution of ultra pure acrylamide and bis-acrylamide in deionised water. The gel is formed by the polymerisation of the acrylamide monomer with a co-monomer often N,N'-Methylene-bis-acrylamide, which acts as a cross-linking agent, in the presence of free radicals or a photochemical initiator.
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Agarose LE, molecular biology grade, Axygen®
Supplier: Corning
Axygen® Agarose Low Electroendosmosis (LE) is a high quality molecular biology grade agarose suitable for analytical and preparative electrophoresis of nucleic acids.
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Agarose, genetic technology grade, MP Biomedicals
Supplier: MP Biomedicals
Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae.