Nucleic Acid Reagents

Supplier: MP Biomedicals

SPINeasy PCR purification and gel extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis.

Supplier: Thermo Fisher Scientific

A combined mixture of  dATP, dCTP, dGTP and dTTP containing 5 mM of each dNTP. Applications include PCR, RT-PCR, DNA labelling, and site-directed mutagenesis.

Supplier: Cytiva

T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.

Supplier: Corning

The AxyPrep™ Mag Tissue gDNA Kit utilises a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. DNA from other tissue types may be extracted with this protocol, but optimisation may be required.

Supplier: MP Biomedicals

SPINeasy Plasmid Midiprep kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 ml of transformed bacterial (E.coli) culture.

Supplier: BIOPREMIER

The RNA viral extraction kit is designed for the rapid simultaneous purification of viral RNA from cell–free samples, solid samples, swabs and transport media.

Supplier: MP Biomedicals

Humic acids, heavy metals, heme are the most notorious PCR inhibitors. This could be due to non-optimised DNA purification procedures which often co-purify inhibitors and lead to false negative results on downstream applications.

Supplier: EKSIGENT

The DNA 20 kb plasmid and linear kit is used to do isoform and purity analysis of plasmid and linearised dsDNA.

Supplier: Corning

Axygen power supplies are ideal for most electrophoresis needs.

Supplier: Cytiva

High purity dNTPs for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers. Versions are available for use with probes or with SYBR® Green I included, and with or without ROX or Fluorescein passive reference dyes.

Supplier: Cytiva

illustra™ dNTPs are high purity solutions for enzymatically sensitive applications, such as nucleic acid amplification, sequencing, labelling, mutagenesis, cDNA synthesis and expression profiling.

Supplier: Cytiva

illustra™ Ready-To-Go™ GenomiPhi™ HY DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 60 μg yield from nanogram amounts of DNA sample.

Supplier: MP Biomedicals

MagBeads FastDNA® Kit for Blood is intended for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Supplier: Cytiva

Sera-Mag Select PCR clean-up, size selection reagent combines the exceptional binding characteristics of Sera-Mag carboxyl speedbeads with an optimised binding solution in a ready to use formulation. The DNA size range isolated from the process can be tailored to suit the user’s end requirements by adjusting the amount of reagent that is mixed with a fixed volume of sample.

Supplier: Cytiva

RNAspin Mini RNA Isolation Kit is a complete RNA purification kit designed for rapid extraction of high-quality total RNA from a wide range of sample types.