Nucleic Acid Reagents

Supplier: Cytiva

ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.

Supplier: Cytiva

illustra™ ExoProStar™ contains illustra™ Alkaline Phosphatase and Exonuclease 1, formulated to work together to remove unincorporated primers and nucleotides from amplification reactions in preparation for sequencing, cloning, genotyping or further DNA modification reactions.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Supplier: Cytiva

The illustra™ GenomiPhi™ HY DNA Amplification Kit is part of the Phi29 DNA polymerase family of products. It contains all of the components necessary for genomic DNA preparation by isothermal strand displacement amplification. The product protocol is very simple, and provides typical yields of 40 to 50 μg DNA and average product lengths 10 kb. The starting material for GenomiPhi reactions can be purified DNA or non purified cell lysates.

Supplier: Cytiva

The illustra™ plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Produces high quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.

Supplier: G-Biosciences

SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.

Supplier: G-Biosciences

SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.

Supplier: Cytiva

illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

Supplier: Cytiva

High purity dNTPs for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers. Versions are available for use with probes or with SYBR® Green I included, and with or without ROX or Fluorescein passive reference dyes.

Supplier: Cytiva

RNAspin Mini RNA Isolation Kit is a complete RNA purification kit designed for rapid extraction of high-quality total RNA from a wide range of sample types.

Supplier: Cytiva

illustra™ dNTPs are high purity solutions for enzymatically sensitive applications, such as nucleic acid amplification, sequencing, labelling, mutagenesis, cDNA synthesis and expression profiling.

Supplier: Cytiva

Sera-Mag Select PCR clean-up, size selection reagent combines the exceptional binding characteristics of Sera-Mag carboxyl speedbeads with an optimised binding solution in a ready to use formulation. The DNA size range isolated from the process can be tailored to suit the user’s end requirements by adjusting the amount of reagent that is mixed with a fixed volume of sample.

Supplier: MP Biomedicals

The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungi, plant and animal genomic DNA from soil and environmental samples.

Supplier: Cytiva

illustra™ Ready-To-Go™ GenomiPhi™ HY DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 60 μg yield from nanogram amounts of DNA sample.

Supplier: MP Biomedicals

MagBeads FastDNA® Kit for Blood is intended for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.