You searched for: Nucleic Acid Reagents
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
RNA isolation, total RNA kit II, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
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E.Z.N.A.® Blood DNA Kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® blood DNA kit provides rapid total DNA isolation from up to 250 µl of fresh or frozen anticoagulated whole blood. The E.Z.N.A.® blood DNA kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® blood DNA method is ready for applications such as PCR, southern blotting, or restriction enzyme digestion.
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Tissue DNA kit, genomic DNA isolation from tissue, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® tissue DNA kit offers a simple, rapid, and cost effective method for the isolation of DNA from a wide variety of sample sources including fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in less than 30 minutes. Up to 30 mg of tissue at a time can be readily processed at a time using the simple E.Z.N.A.® tissue DNA protocol. Single or multiple samples can be simultaneously processed with this spin-column based kit. There is no need for phenol/chloroform extractions, or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® tissue DNA kit is ready for most downstream applications such as PCR, southern blot and restriction enzyme digestion.
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RNA isolation, total RNA kit, E.Z.N.A.® MicroElute
Supplier: OMEGA BIO-TEK
E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.
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E.Z.N.A.® PX Blood RNA Kit
Supplier: OMEGA BIO-TEK
Isolates total RNA from blood samples stored in special preservation reagents and PAXgene tubes using spin columns.
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Chemicon® BacterialXpress™ Nucleic Acid Extraction Kit
Supplier: Merck
BacterialXpress™ Nucleic acid extraction kit is intended for the purification of bacterial DNA from plasma, serum, cerebral spinal fluid, amniotic fluid, tissue, bone marrow, and cell culture.
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E-Z Select® Robotic Reservoir, 600 ml
Supplier: OMEGA BIO-TEK
The E-Z Select® Robotic Reservoir, 600 ml is designed for holding large volume reagent for use on automated platforms such as Hamilton Microlab STAR.
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Mag-Bind® Total RNA 96 kit
Supplier: OMEGA BIO-TEK
Isolate total cellular RNA and viral RNA from a wide variety of cells and tissues.
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E.Z.N.A.® HP Plant & Fungal DNA Kit
Supplier: OMEGA BIO-TEK
Isolate DNA from plant or fungal sample with organic solvents using spin columns.
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Mag-Bind® cfDNA kit
Supplier: OMEGA BIO-TEK
The Mag-Bind® cfDNA Kit is designed for rapid and reliable isolation of circulating DNA from 500 to 4000 µl plasma or serum samples. The Mag-Bind® cfDNA Kit can be processed manually or with automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.
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Plasmid isolation, single-strand phage DNA, M-13 isolation kits, E.Z.N.A.® and E-Z 96®
Supplier: OMEGA BIO-TEK
E.Z.N.A.® M13 DNA kits are designed to purify up to 10 μg of single-stranded DNA from up to 3 ml of phage supernatant. Yields of single-stranded DNA obtained using E.Z.N.A.® M13 DNA kits are around 3 to 10 μg and reproducible when the isolations are performed from the same culture. Kits are also available in E-Z 96® DNA plate format.
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AdEasy virus purification kit
Supplier: AGILENT
The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution. Concentrated and purified viral particles can be obtained in 2 to 3 hours, in contrast to traditional CsCl gradient centrifugation which typically takes 12 to 48 hours.
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Mag-Bind® Endo-free Plasmid Mini Kit
Supplier: OMEGA BIO-TEK
The Mag-Bind® Endo-free Plasmid Mini Kit is designed to deliver high-quality, plasmid DNA with endotoxin levels <0,1 EU/μg for use in eukaryotic transfections and other sensitive in vitro applications.
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Universal pathogen DNA kit, Mag-Bind®
Supplier: OMEGA BIO-TEK
The Mag-Bind® Universal Pathogen DNA 96 Kit allows rapid and reliable isolation of high-quality pathogen and host genomic DNA from tissue samples. Up to a 30 mg tissue sample is recommended and can be processed in less than 60 minutes. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.
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RNA isolation, total RNA kit, E-Z 96®
Supplier: OMEGA BIO-TEK
The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.
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Genomic DNA purification kit, Fermentas
Supplier: Thermo Fisher Scientific
DNA purification kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
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Viral RNA Xpress kit, Mag-Bind®
Supplier: OMEGA BIO-TEK
Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.
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Montage® SEQ96 Sequencing Reaction Cleanup Kits
Supplier: Merck
The Montage® SEQ96 sequencing reaction cleanup kit provides the filtration plate and solution necessary to remove contaminating salts and unincorporated dye terminators from DNA sequencing reactions.
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cfDNA DNA kit, CE IVD, Mag-Bind®
Supplier: OMEGA BIO-TEK
The Mag-Bind® cfDNA Kit CE IVD is designed for rapid and reliable isolation of circulating cell-free DNA from 1 to 4 ml plasma/serum samples.
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Tissue DNA kit, genomic DNA isolation from tissue, E-Z 96®
Supplier: OMEGA BIO-TEK
By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.
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Mag-Bind® Plant DNA DS 96 kit
Supplier: OMEGA BIO-TEK
The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.
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E.Z.N.A.® Plasmid Midi kit
Supplier: OMEGA BIO-TEK
E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.
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NUCLEIC-CARD™ Nucleic Acid Stabilisation and Archiving Devices
Supplier: Copan
NUCLEIC-CARD™ is specifically designed to collect, transport, and store human DNA from buccal cells, saliva, blood, etc. Thanks to the unique chemical treatment, NUCLEIC-CARD™ preserves DNA for 20 years at room temperature.
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gDNA Removal kit
Supplier: Enzo Life Sciences
Rapid and complete removal of gDNA from RNA preps prior to reverse transcription.
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E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)
Supplier: OMEGA BIO-TEK
Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.
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Resin, StrataClean
Supplier: AGILENT
StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible.
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E.Z.N.A.® Tissue DNA Kit, E.Z.N.A®
Supplier: OMEGA BIO-TEK
Isolate DNA from tissues, buccal swabs, cultured cells, whole blood, body fluids, paraffin-embedded tissues, and mouse tail snips using mini spin columns.
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EX-WAX™ Paraffin-Embedded DNA Extraction Kit
Supplier: Merck
CHEMICON's EX-WAX™ DNA Extraction Kit is intended to extract DNA from paraffin-embedded tissue fixed in 10% Formalin or non-crosslinking fixatives. DNA extracted by this kit is suitable for amplification by PCR.
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E-Z Select® 24-Well Plate, 25 ml
Supplier: OMEGA BIO-TEK
E-Z Select® 24-well Plate, 25 ml is designed for performing large volume applications on automated platforms such as Hamilton Microlab® STAR or equivalent.
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Mag-Bind® DNA/RNA Kit
Supplier: OMEGA BIO-TEK
High-throughput DNA and RNA isolation from cultured cells and tissue using magnetic beads.