You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade
Supplier: VWR Chemicals
Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)
Expand 1 Items
VWR® Phosphate Buffered Saline (PBS), Low Phosphate
Supplier: VWR Chemicals
Irradiated solution for use in washing cells.
Expand 1 Items
VWR® Buffer, MES-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
Expand 1 Items
Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
Expand 1 Items
Phosphate buffered saline (PBS), 10X
Supplier: G-Biosciences
PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.
Expand 1 Items
Tris-Borate-EDTA (TBE) buffer, 10X
Supplier: PanReac AppliChem
10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.
Expand 2 Items
Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
Expand 15 Items
VWR®, Blocking buffer, non-fat powdered milk
Supplier: VWR Chemicals
Each pack of powdered blocking buffer makes one liter of 1X solution.
Expand 1 Items
VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20
Supplier: VWR Chemicals
Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.
Expand 1 Items
VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade
Supplier: VWR Chemicals
PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.
Expand 1 Items
Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
Expand 1 Items
IHC Wash Buffer, Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
Expand 1 Items
VWR®RapidBlock™, Blocking solution, 10x
Supplier: VWR Chemicals
RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.
Expand 1 Items
Buffer, PBS solution 10X concentrate (phosphate buffered saline)
Supplier: G-Biosciences
10X PBS (Phosphate Buffered Saline) is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer and saline solution, which when diluted 1 to 10 in ultrapure water produces a phosphate buffered saline of 8mM NaH2PO4, 150mM NaCl, 3mM KCl, and 2mM KH2PO4 at pH 7.4.
Expand 2 Items
Non-animal protein blocking Agent, NAP-BLOCKER™
Supplier: G-Biosciences
NAP-BLOCKER™ is an animal-free protein blocking agent recommended for improved assay sensitivity, minimal non specific binding and a high signal-to-background ratio. It is suitable for Western blots, dot blots and assay development.
Expand 3 Items
Transfer buffer, Tris-glycine,10X, AccuGENE™
Supplier: LONZA
AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.
Expand 1 Items
PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
Expand 1 Items
Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: BIOMOL RESEARCH LABORATORIES
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
Expand 2 Items
Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
Expand 1 Items
Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
Expand 1 Items
In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
Expand 1 Items
6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
Expand 1 Items
Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
Expand 1 Items
Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
Expand 4 Items
Blocking buffers, Protein-Free™
Supplier: G-Biosciences
Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
Expand 4 Items
Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
Expand 1 Items
SSC buffer, 20X concentrate, pH 7,0
Supplier: Rockland Immunochemicals
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
Expand 1 Items
Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: BIOMOL RESEARCH LABORATORIES
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
Expand 2 Items
Dry buffer packs, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.
Expand 4 Items
TPE buffer, 10X tris-phosphate-EDTA, pH 8
Supplier: Rockland Immunochemicals
Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)