Cell Culture Buffers
Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.
EDTA, 0.5M pH 8.0, Corning®
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.
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10X Tris buffered saline, liquid, Corning®
Supplier: Corning
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.
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Krebs-Ringer solution, bicarbonate-buffered
Supplier: Thermo Fisher Scientific
Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.
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TE buffer, pH 8,1, RNAse free
Supplier: Thermo Fisher Scientific
Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.
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Sodium bicarbonate, Corning®
Supplier: Corning
Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment
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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™
Supplier: HyClone products (Cytiva)
HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.
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Phosphate-buffered saline (PBS, 10X), pH 7,6
Supplier: Thermo Fisher Scientific
Cell culture buffer, PBS, 10 X
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HEPES-buffered saline, pH 7,0 (2X for transfection)
Supplier: Thermo Fisher Scientific
Cell culture buffer, HEPES-buffered Saline
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Phosphate-buffered saline (PBS, 10X), RNase free
Supplier: Thermo Fisher Scientific
Cell culture buffer, PBS, 10 X, RNAse free
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Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, without calcium, without magnesium
Supplier: Thermo Fisher Scientific
Cell culture buffer, DPBS, 1X
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Carbonate-buffered saline (5X), pH 9,5
Supplier: Thermo Fisher Scientific
Cell culture buffer, Carbonate-buffered Salin, 5X
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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)
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Hank's balanced salt solution, modified, MP Biomedicals
Supplier: MP Biomedicals
Cell culture buffer, Hank's balanced salt solution, modified (1X)
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals
Supplier: MP Biomedicals
Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.
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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals
Supplier: MP Biomedicals
Used to minimise cell attachment or aggregation.