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Protein Purification

Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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Protein A Agarose (High Affinity), Abcam

Supplier: Abcam

Protein A Agarose (High Affinity) (ab193255) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Antibody Purification Kit (Protein G), Abcam

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128747 quickly removes these contaminants.

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Unblocked Protein A Beads, Abcam

Supplier: Abcam

Abcam's Unblocked protein A beads are agarose beads that have been extensively validated in chromatin immunoprecipitation assays (ChIP).

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Chicken affinity purification resin

Chicken affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Chicken IgY Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Chicken IgY from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Protein A and G Antibody Purification Kits

Protein A and G Antibody Purification Kits

Supplier: G-Biosciences

Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

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Protein purification kits, HOOK™

Protein purification kits, HOOK™

Supplier: G-Biosciences

For the rapid purification of soluble, 6X His or GST tagged proteins.

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Protein purification kit, STRATEGY™

Protein purification kit, STRATEGY™

Supplier: G-Biosciences

STRATEGY™ is designed to allow researchers to rapidly screen a number of purification techniques in a small scale format to identify the system to isolate their protein of interest. The self-contained kit allows researchers to save time and money as separate buffers, resins and columns are not needed.

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Amicon® Pro Purification System

Amicon® Pro Purification System

Supplier: Merck

The Amicon® Pro purification system - A centrifugal tool collapsing protein purifcation with concentration and buffer exchange.

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Magnetic Antibody Purification System, Abcam

Supplier: Abcam

Magnetic Antibody Purification System (ab270532) is a convenient method to purify antibodies at a small scale with high recoveries.

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TCS Antibody Purification Kit, Abcam

Supplier: Abcam

TCS Antibody Purification Kit (ab109207) has been designed for tissue culture supernatant purification, and can be used to purify 10-50 mL in each purification.

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Mouse Antibody Purification Kit (Gold/Europium labelling), Abcam

Supplier: Abcam

Mouse Antibody Purification Kit - Nanoparticles (ab204910) removes contaminants often found in commercial antibodies.

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Proteomic protein controls

Supplier: G-Biosciences

Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, E.coli, yeast, plant or as a control set that contains one 2 mg vial of each.

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Sulfhydryl coupling resin for antibody purification

Sulfhydryl coupling resin for antibody purification

Supplier: G-Biosciences

This sulphhydryl coupling resin is designed for coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH). The iodoacetyl groups of the sulfhydryl coupling resin specifically react with free sulfhydryls to form covalent, permanent thioether bonds.

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Affinity chromatography resin, chelating nickel

Affinity chromatography resin, chelating nickel

Supplier: G-Biosciences

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

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Immobilised D-galactose for lectin purification

Immobilised D-galactose for lectin purification

Supplier: G-Biosciences

Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.

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Diluent M Concentrate (10x)

Diluent M Concentrate (10x)

Supplier: Abcam

Diluent M Concentrate (10X) (ab138908) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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