You searched for: Protein Purification

Supplier: G-Biosciences

For the rapid purification of soluble, 6X His or GST tagged proteins.

Supplier: G-Biosciences

Cobalt chelating resin, for the purification of 6X His proteins

Supplier: Avantor

The J.T.Baker^® BAKERBOND^® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

Supplier: Avantor

J.T.Baker® BAKERBOND® protein precipitation plates enable fast protein removal from biological samples.

Supplier: Avantor

Silica-based media used for the purification of proteins based on differential salt induced hydrophobicity. Mixed mode provides unique selectivity with its hydrophobic interaction and ion exchange function.

Supplier: Merck

The Amicon® Pro purification system - A centrifugal tool collapsing protein purifcation with concentration and buffer exchange.

Supplier: G-Biosciences

STRATEGY™ is designed to allow researchers to rapidly screen a number of purification techniques in a small scale format to identify the system to isolate their protein of interest. The self-contained kit allows researchers to save time and money as separate buffers, resins and columns are not needed.

Supplier: G-Biosciences

This avidin resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labelled antibodies.

Supplier: G-Biosciences

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Supplier: G-Biosciences

Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.

Supplier: G-Biosciences

Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

Supplier: G-Biosciences

The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: Genscript

Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.

Supplier: G-Biosciences

Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

Supplier: G-Biosciences

The Pearl™ IgG purification resin allows for the one-step purification of immunoglobulin G from serum. The resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: ImmunoReagents

ImmunoReagents Mouse IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Mouse IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: Avantor

J.T.Baker® BAKERBOND® supported liquid extraction (SLE) plates are an ideal and faster alternative to traditional liquid-liquid extraction methods and allow excellent recoveries of analytes from biological matrices.

Supplier: ImmunoReagents

ImmunoReagents Chicken IgY Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Chicken IgY from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: G-Biosciences

This sulphhydryl coupling resin is designed for coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH). The iodoacetyl groups of the sulfhydryl coupling resin specifically react with free sulfhydryls to form covalent, permanent thioether bonds.

Supplier: ENZO LIFE SCIENCES

Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.

Supplier: ImmunoReagents

ImmunoReagents Goat IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Goat IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: G-Biosciences

The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

Supplier: G-Biosciences

Immobilised Pepstatin is for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin.

Supplier: G-Biosciences

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Supplier: G-Biosciences

Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Supplier: ImmunoReagents

ImmunoReagents Rabbit IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Rabbit IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: ImmunoReagents

ImmunoReagents Sheep IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Sheep IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: Rockland Immunochemicals

Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.

Supplier: G-Biosciences

The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: AGILENT

The affinity removal spin cartridge reagent kit is designed to remove the most interfering high-abundant protein (albumin) from human samples (such as plasma, serum, urine or cerebrospinal fluid).