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You searched for: Protein Purification Resins

Gel filtration media, Superdex™ 30 PG

Gel filtration media, Superdex™ 30 PG

Supplier: Cytiva

Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose

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CM Sephadex™ C-25 Ion Exchange Chromatography Media

CM Sephadex™ C-25 Ion Exchange Chromatography Media

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)

Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)

Supplier: Cytiva

Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.

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Ion exchange chromatography media, SOURCE™ 30S

Ion exchange chromatography media, SOURCE™ 30S

Supplier: Cytiva

SOURCE 30S is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix It is modified with sulphonate (S) strong cation exchange groups.

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Reversed phase chromatography media, SOURCE™ 15RPC

Reversed phase chromatography media, SOURCE™ 15RPC

Supplier: Cytiva

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.

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Affinity chromatography media, Ni Sepharose™ 6 Fast Flow

Affinity chromatography media, Ni Sepharose™ 6 Fast Flow

Supplier: Cytiva

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

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Gel filtration media, Sephadex™ G-100

Gel filtration media, Sephadex™ G-100

Supplier: Cytiva

Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.

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Affinity coupling media, EAH Sepharose™ 4B

Affinity coupling media, EAH Sepharose™ 4B

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

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Gel filtration calibration kits

Gel filtration calibration kits

Supplier: Cytiva

Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns, such as Superdex, Superose and Sephacryl.

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Gelatin-Sepharose® 4B

Gelatin-Sepharose® 4B

Supplier: Cytiva

Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.

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Capto™ adhere ImpRes multimodal chromatography media, Cytiva

Capto™ adhere ImpRes multimodal chromatography media, Cytiva

Supplier: Cytiva

Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.

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Affinity chromatography media, Streptavidin Sepharose™ High Performance

Affinity chromatography media, Streptavidin Sepharose™ High Performance

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

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Sepharose™ CL-6B Gel Filtration Base Matrix

Sepharose™ CL-6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Sepharose™ 6B Gel Filtration Base Matrix

Sepharose™ 6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Affinity chromatography media, MabSelect Xtra™

Affinity chromatography media, MabSelect Xtra™

Supplier: Cytiva

MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.

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Gel filtration media, Sephadex™ G-10

Gel filtration media, Sephadex™ G-10

Supplier: Cytiva

Sephadex™ G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.

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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media

Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity

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Ion exchange chromatography media, CM Sepharose™ Fast Flow

Ion exchange chromatography media, CM Sepharose™ Fast Flow

Supplier: Cytiva

CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow

Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow

Supplier: Cytiva

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.

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Affinity chromatography media, Chelating Sepharose™ Fast Flow

Affinity chromatography media, Chelating Sepharose™ Fast Flow

Supplier: Cytiva

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

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Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.

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Sepharose™ CL-4B Gel Filtration Base Matrix

Sepharose™ CL-4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-4B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Affinity chromatography media, Glutathione Sepharose™ 4B

Affinity chromatography media, Glutathione Sepharose™ 4B

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

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Affinity chromatography media, Blue Sepharose™ High Performance

Supplier: Cytiva

This media is suitable for affinity chromatography.

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Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Supplier: Cytiva

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

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Butyl Sepharose™ High Performance Hydrophobic interaction chromatography media

Butyl Sepharose™ High Performance Hydrophobic interaction chromatography media

Supplier: Cytiva

Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.

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Affinity chromatography media, Ni Sepharose™ High Performance

Affinity chromatography media, Ni Sepharose™ High Performance

Supplier: Cytiva

Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).

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Affinity chromatography media, MabSelect SuRe™

Affinity chromatography media, MabSelect SuRe™

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Ion exchange chromatography media, DEAE Sephacel™

Ion exchange chromatography media, DEAE Sephacel™

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

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Affinity purification media, rProtein A Sepharose™ Fast Flow

Affinity purification media, rProtein A Sepharose™ Fast Flow

Supplier: Cytiva

rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.

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