Protein Purification Resins
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Affinity purification media, rProtein A Sepharose™ Fast Flow
Supplier: Cytiva
rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.
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Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
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Affinity chromatography media, Ni Sepharose™ High Performance
Supplier: Cytiva
Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).
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Affinity chromatography media, Glutathione Sepharose™ High Performance
Supplier: Cytiva
Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.
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Affinity chromatography media, Ni Sepharose™ 6 Fast Flow
Supplier: Cytiva
Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.
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2D Clean-Up kit
Supplier: Cytiva
The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.
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SDS-PAGE Clean Up Kit
Supplier: Cytiva
SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.
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Affinity chromatography media, MabSelect Xtra™
Supplier: Cytiva
MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.
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Ion exchange chromatography media, Capto™ Q
Supplier: Cytiva
Capto™ Q is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Affinity chromatography media, MabSelect SuRe™
Supplier: Cytiva
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
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Butyl-S Sepharose™ 6 Fast Flow Hydrophobic interaction chromatography media
Supplier: Cytiva
Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.
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Ion exchange chromatography media, Capto™ S
Supplier: Cytiva
Capto™ S is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Gel filtration calibration kits
Supplier: Cytiva
Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns, such as Superdex, Superose and Sephacryl.
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Affinity chromatography media, IMAC Sepharose™ High Performance
Supplier: Cytiva
IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
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Affinity chromatography media, Dextrin Sepharose™ High Performance
Supplier: Cytiva
Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).
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SP Sepharose™ XL Ion Exchange Chromatography Media
Supplier: Cytiva
SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.