You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Choline Quantitation Kit
Supplier: AAT Bioquest
Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis).
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ReadiUse™ NADP Regenerating Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.
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Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*
Supplier: AAT Bioquest
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Amplite® Blue Peroxidase Substrate
Supplier: AAT Bioquest
A variety of substrates are available for detecting peroxidase activity in ELISA assays. colourimetric HRP substrates (e.g., TMB, OPD and ABTS) have been widely used for years. Each of these substrates varies greatly with respect to its performance characteristics such as detection sensitivity, working range and attainable signal-to-noise ratios. Amplite® Blue peroxidase substrate is a soluble fluorogenic substrate for detection of peroxidase activity. Amplite® Blue allows rapid HRP detection assays to be performed with greater sensitivity than the colourimetric HRP substrates (such as TMB and OPD). The fluorescent product of Amplite® Blue does not photobleach. Amplite® Blue exhibits a flat baseline in assays, which facilitates low-level detection sensitivity and allows for high signal-to-noise ratios.
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CPRG assay kit
Supplier: OZ BIOSCIENCES
CPRG assay kit is designed to quantify low expression level β-Galactosidase.
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Amplite® Colorimetric Xanthine Oxidase Assay Kit
Supplier: AAT Bioquest
Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyse the oxidation of xanthine to uric acid.
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Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen.
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Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
Supplier: AAT Bioquest
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.
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Amplite® Fluorimetric cADP-Ribose Assay Kit
Supplier: AAT Bioquest
This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.
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Amplite® Fluorimetric Xanthine Assay Kit
Supplier: AAT Bioquest
Xanthine is a purine base found in most human body tissues and fluids.
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Amplite® Colorimetric Xanthine Assay Kit
Supplier: AAT Bioquest
Xanthine is a purine base found in most human body tissues and fluids.
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Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit
Supplier: AAT Bioquest
Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.
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Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit
Supplier: AAT Bioquest
Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.
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Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit
Supplier: AAT Bioquest
Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.
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Amplite® Fluorimetric D-Lactate Assay Kit
Supplier: AAT Bioquest
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
Supplier: AAT Bioquest
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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Amplite® Fluorimetric DPP4 Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® fluorimetric DPP4 activity assay kit provides a simple, quick, and direct protocol for measuring DPP4 Activity.
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Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit
Supplier: AAT Bioquest
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
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MMP Red™ Substrate
Supplier: AAT Bioquest
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Colorimetric Glucose Oxidase Assay Kit
Supplier: AAT Bioquest
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.
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Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT Bioquest
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Gama-Glutamyltransferase (GGT) Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Colorimetric Gamma-Glutamyltransferase (GGT) Activity Assay Kit provides an efficient and straightforward method for measuring GGT activity across various sample types.
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Pierce™ Cypridina Luciferase Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
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Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® colorimetric GAPDH activity assay kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalysed by GAPDH.
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Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*
Supplier: AAT Bioquest
Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.
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Amplite® Fluorimetric L-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT Bioquest
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit
Supplier: AAT Bioquest
Lead is a highly poisonous metal to human beings, especially young children.
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Gaussia luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.