You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric Glucose Oxidase Assay Kit
Supplier: AAT Bioquest
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.
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Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT Bioquest
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Gama-Glutamyltransferase (GGT) Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Colorimetric Gamma-Glutamyltransferase (GGT) Activity Assay Kit provides an efficient and straightforward method for measuring GGT activity across various sample types.
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Pierce™ Cypridina Luciferase Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
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Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® colorimetric GAPDH activity assay kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalysed by GAPDH.
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Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*
Supplier: AAT Bioquest
Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.
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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit
Supplier: AAT Bioquest
Lead is a highly poisonous metal to human beings, especially young children.
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Gaussia luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.
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Amplite® Fluorimetric Endotoxin Detection Kit
Supplier: AAT Bioquest
Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria.
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Amplite® Rapid Colorimetric Maleimide Quantitation Kit
Supplier: AAT Bioquest
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules.
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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.
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Pierce™ Alkaline Phosphatase, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.
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Pierce™ Firefly Luc One-Step Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.
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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit
Supplier: AAT Bioquest
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.
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Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways.
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Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative.
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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.
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Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit
Supplier: AAT Bioquest
Superoxide dismutases (SOD) are a class of enzymes that catalyse the dismutation of superoxide into oxygen and hydrogen peroxide.
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Amplite® Fluorimetric Melanin Assay Kit
Supplier: AAT Bioquest
Melanins have very diverse roles and functions in various organisms.
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Amplite® Luminometric Nitroreductase Assay Kit
Supplier: AAT Bioquest
Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds.
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Amplite® Colorimetric D-Lactate Assay Kit
Supplier: AAT Bioquest
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Ascorbic Acid Assay Kit
Supplier: AAT Bioquest
L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.
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Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT Bioquest
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Fructose-6-Phosphate Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® colorimetric fructose-6-phosphate assay kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples.
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Quantitative peroxide assay kit (aqueous), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT Bioquest
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.
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Phosphatase assay kit
Supplier: G-Biosciences
The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.
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Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*
Supplier: AAT Bioquest
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.