You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Fluorimetric Lead (II) Ion Quantitation Kit
Supplier: AAT Bioquest
Lead is a highly poisonous metal to human beings, especially young children.
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Gaussia luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.
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Amplite® Fluorimetric Endotoxin Detection Kit
Supplier: AAT Bioquest
Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria.
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Amplite® Rapid Colorimetric Maleimide Quantitation Kit
Supplier: AAT Bioquest
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules.
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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.
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Pierce™ Alkaline Phosphatase, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.
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Pierce™ Firefly Luc One-Step Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.
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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit
Supplier: AAT Bioquest
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.
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Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways.
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Amplite® Colorimetric Beta-Lactamase Activity Assay Kit
Supplier: AAT Bioquest
β-lactamases are a large family of enzymes capable of hydrolysing β-lactams.
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Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative.
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Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT Bioquest
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.
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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.
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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.
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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.
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Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.
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Amplite® Colorimetric Enterokinase Activity Assay Kit
Supplier: AAT Bioquest
Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.
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Amplite® Colorimetric Pyruvate Assay Kit
Supplier: AAT Bioquest
Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Colorimetric Maleimide Quantitation Kit
Supplier: AAT Bioquest
Maleimides can be directly assayed spectrophotometrically at 302 nm.
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Amplite® Colorimetric L-Alanine Assay Kit
Supplier: AAT Bioquest
L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Amplite® Colorimetric Glycerol Assay Kit
Supplier: AAT Bioquest
Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue.
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Amplite® Colorimetric BCA Protein Quantitation Assay Kit
Supplier: AAT Bioquest
The traditional BCA protein assay is widely used for quantifying proteins.
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Amplite® Colorimetric Glucose-6-Phosphate Assay Kit
Supplier: AAT Bioquest
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.
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Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Caspases play important roles in apoptosis and cell signaling.
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Amplite® Fluorimetric L-Lactate Assay Kit
Supplier: AAT Bioquest
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.
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Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.