Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
PTP1B drug discovery kit
Supplier: Enzo Life Sciences
The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.
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Cathepsin S drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The Cathepsin S Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin S. Using a convenient microplate format, cathepsin S activity is monitored fluorimetrically using the substrate provided in the kit.
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Glutathione peroxidase activity kit
Supplier: Enzo Life Sciences
The Glutathione Peroxidase activity kit is a convenient 96-well plate based assay for the higher throughput analysis of glutathione peroxidase activity in a variety of sample matrices in just 30 minutes.
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Fluorometric drug discovery assay kit, Green SIRT5, Fluor-de-Lys®
Supplier: Enzo Life Sciences
FLUOR DE LYS® Green SIRT5 fluorometric drug discovery kit is a sensitive fluorometric assay that measures the lysyl desuccinylase activity of the recombinant human SIRT5.
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Matrix Metalloproteinase-13 (MMP-13) colorimetric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-13 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a thiopeptide as a chromogenic substrate.
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Matrix Metalloproteinase-20 (MMP-20) colorimetric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-20 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-20 (MMP-20, enamelysin) using a thiopeptide as a chromogenic substrate.
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Protease assay kit
Supplier: G-Biosciences
This Protease Assay Kit is designed for the quantitative determination of proteases present in protein sample, using a dye-labelled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labelled peptides. The absorbance of the dye-labelled peptide is measured at 570 nm for determination of protease activity.
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Carbonic Anhydrase (CA) Inhibitor Screening Kit (Colorimetric)
Supplier: Abcam
It is simple and can be used to identify and characterize CA inhibitors in a high-throughput format.
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Matrix Metalloproteinase-14 (MMP-14) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-14 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-14 (MMP-14, membrane-type matrix metalloproteinase 1, MT1-MMP) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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SAM Methyltransferase assay, SAMfluoro™
Supplier: G-Biosciences
The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit provides a simple and direct procedure for precise measurement of PFK enzyme activity in various biological samples.
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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*
Supplier: AAT Bioquest
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
Supplier: AAT Bioquest
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Amplite® Colorimetric Acetylcholinesterase Assay Kit
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit
Supplier: AAT Bioquest
The traditional Bradford protein assay is widely used for quantifying protein concentrations.
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Amplite® Fluorimetric NAD Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.
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Amplite® Fluorimetric L-Alanine Assay Kit
Supplier: AAT Bioquest
L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
E. coli beta-galactosidase is a 464 Kd tetramer.
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Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
Monitoring of various protease activities has become a routine task for many biological laboratories.
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Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit
Supplier: AAT Bioquest
Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized liver and present in blood.
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Amplite® Colorimetric NADP/NADPH Ratio Assay Kit
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric Ammonia Quantitation Kit *Blue Color*
Supplier: AAT Bioquest
Ammonia is an important source of nitrogen for living systems.
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Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® fluorimetric neutrophil elastase activity assay kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity.
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Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Hydrogen peroxide (H₂O₂) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.
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Amplite® Colorimetric Urea Quantitation Kit *Blue Color*
Supplier: AAT Bioquest
Urea is the final degradation product of protein and amino acid metabolism in animals.
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Amplite® Colorimetric Zinc Ion Quantitation Kit
Supplier: AAT Bioquest
Zinc is an essential trace mineral element that plays an important role in a number of biological processes.
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Amplite® Colorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT Bioquest
Alpha-ketoglutarate (α-Ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.