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You searched for: Cellular Assays

Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

ADAM17 fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.

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Yellow IHC chromogen (HRP), HighDef®

Supplier: ENZO LIFE SCIENCES

Yellow IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

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Red IHC chromogen (AP), HIGHDEF®

Supplier: ENZO LIFE SCIENCES

Red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.

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p70 S6K activity kit

Supplier: ENZO LIFE SCIENCES

The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

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qScript® ultra flex kits

qScript® ultra flex kits

Supplier: Quantabio

Faster, longer, tougher next gen cDNA synthesis with flexible priming.

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Blue IHC chromogen (HRP), HIGHDEF®

Supplier: ENZO LIFE SCIENCES

Blue IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

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CV-Cathepsin L detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.

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ROS-ID™ NO Detection kit

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.

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FLUOR DE LYS® HDAC fluorometric activity assay kit

Supplier: ENZO LIFE SCIENCES

The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.

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Solutions colorantes, Ghost Dye™

Solutions colorantes, Ghost Dye™

Supplier: Tonbo Biosciences

Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.

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Catalase fluorometric detection kit

Supplier: ENZO LIFE SCIENCES

The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.

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LEADING LIGHT® Wnt cell line assay reagents

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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Green long-term cell tracer, CYTO-ID®

Supplier: ENZO LIFE SCIENCES

Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

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ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit

Supplier: ENZO LIFE SCIENCES

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

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CV-Cathepsin B detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

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Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Supplier: AAT BIOQUEST

Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).

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Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence*

Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells.

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Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*

Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells.

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LEADING LIGHT® Wnt Reporter Assay Starter Kit

Supplier: ENZO LIFE SCIENCES

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

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Caspase-3 assay kits, NucView™ 488

Caspase-3 assay kits, NucView™ 488

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

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LysoView™ Lysosomal Stain

LysoView™ Lysosomal Stain

Supplier: Biotium

LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.

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Aggresome detection kit, PROTEOSTAT®

Supplier: ENZO LIFE SCIENCES

PROTEOSTAT® Aggresome Detection Kit is a robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology.

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NUCLEAR-ID® green cell cycle kit for flow cytometry

Supplier: ENZO LIFE SCIENCES

For cell cycle results independent of incubation time, temperature, dye and cell concentrations.

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Apoptosis assay kit, NucView® 488 and MitoView™ 633

Apoptosis assay kit, NucView® 488 and MitoView™ 633

Supplier: Biotium

NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

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CF® Dye Dextran 150000 MW, anionic and fixable

CF® Dye Dextran 150000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.

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SREBP-1 transcription factor assay kit

SREBP-1 transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of SREBP-1.

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Bacterial viability and gram stain kit

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

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ROS-ID™ total ROS detection kit

ROS-ID™ total ROS detection kit

Supplier: ENZO LIFE SCIENCES

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.

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CF® Dye Dextran 3500 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.

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Hydrogen peroxide chemiluminescent detection Kit

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of hydrogen peroxide in culture supernatants and cell lysates from any species.

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