You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
VWR® Antibody signal enhancer
Supplier: VWR Chemicals
Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.
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ZnAF-2.tetrahydrochloride
Supplier: ENZO LIFE SCIENCES
Fluorescent probe for Zn2+
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SeramunBlock
Supplier: Apollo Scientific
Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.
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Antibody labelling kits, Mix-n-Stain™ CF™ Dye
Supplier: Biotium
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.
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femtoCHROMO™- AP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
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CF™ dye bovine serum albumin conjugates
Supplier: Biotium
Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.
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Amine reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ NHS-Biotin, 50 mg
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Reactive biotin compounds
Supplier: Biotium
Biotium offers reactive biotin compounds with a variety of reactive functional groups.
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Antibody labelling kits, Mix-n-Stain™ Biotin
Supplier: Biotium
Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.
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AMC (7-Amino-4-methylcoumarin) standard
Supplier: ENZO LIFE SCIENCES
Fluorogenic chromophore. Ex(max): 380nm, Em(max): 460nm. Reference compound.
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Nuclear-ID™ Blue/Red cell viability reagent (GFP-Certified™)
Supplier: ENZO LIFE SCIENCES
Cell viability dye
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femtoCHROMO™- HRP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.
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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates
Supplier: Biotium
Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.
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Mix-n-Stain™ Cyanine dye antibody labelling kits
Supplier: Biotium
Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.
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Annexin V conjugates
Supplier: Biotium
Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.
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HRP - conjugate stabiliser, SeramunStab® STC
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.
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Nick Translation DNA Labelling System 2.0
Supplier: ENZO LIFE SCIENCES
The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent in situ hybridisation (FISH) applications.
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Stabiliser for calibrators and controls, SeramunStab® STD
Supplier: Apollo Scientific
Liquid product yellow odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for complex mixtures of biomolecules.
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CF® Dye dCTP conjugates
Supplier: Biotium
CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.
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Biotin reagents, OneQuant™ HOOK™
Supplier: G-Biosciences
The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.
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CF® Dye hydrazide
Supplier: Biotium
Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.
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CGH labeling kit, CYTAG®
Supplier: ENZO LIFE SCIENCES
Comparative genomic hybridisation (CGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. CGH provides an understanding of genetic disorders, cancers and other genomic aberrations.
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Stabiliser for sensitive biomolecules, SeramunStab® STB
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow, freshly solutions opaleszent, odour weakly pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for sensitive biomolecules like proteohormones or membrane proteins.
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Gold 525 dUTP (lyophilised)
Supplier: ENZO LIFE SCIENCES
Gold 525 dUTP (lyophilised)
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NEDDylation kit
Supplier: ENZO LIFE SCIENCES
This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied). A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.
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CF® Dye cAMP
Supplier: Biotium
Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.
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Carboxyl coupling resin
Supplier: G-Biosciences
Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.
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Green 496 dUTP (lyophilized)
Supplier: ENZO LIFE SCIENCES
Green 496 dUTP (lyophilised)
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Aqua 431 dUTP (lyophilised)
Supplier: ENZO LIFE SCIENCES
Aqua 431 dUTP (lyophilised)