Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
CF®DI Hydrazide
Supplier: Biotium
CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.
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CF®DI Aminooxy
Supplier: Biotium
CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.
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CF™ azide reactive dyes
Supplier: Biotium
CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.
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Cy5® Conjugation Kit (Fast) - Lightning-Link®, Abcam
Supplier: Abcam
Cy5® conjugation / labeling in < 20 mins with 30 secs hands-on time using Cy5® Conjugation Kit (Fast) - Lightning-Link® ab188288. 100% antibody recovery.
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Biotin picolyl azide
Supplier: Biotium
Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
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CF™ dye picolyl azide
Supplier: Biotium
CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.
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Bilirubin conjugate, sodium salt
Supplier: Cayman Chemical
Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.
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Gold Conjugation Kit (40nm, 20 OD), Abcam
Supplier: Abcam
The Abcam Gold Conjugation Kit (ab154873) offers: - Rapid Conjugation: allows for gold conjugation or labeling in less than 4 hours with only 30 seconds of hands-on time. - High Recovery: It ensures 100% antibody recovery, meaning no loss of valuable antibodies. - Stability and Efficiency: The gold nanoparticles have a protective surface coat that provides unrivaled colloidal stability, and the conjugation reaction is initiated simply by reconstituting the freeze-dried nanoparticle
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Gold Conjugation Kit (80nm, 20 OD), Abcam
Supplier: Abcam
80nm Gold conjugation / labeling in < 4 hrs with 30 secs hands-on time using Gold Conjugation Kit (80nm, 20 OD) ab154876. 100% antibody recovery.
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Gold Conjugation Kit (20 nm, 20 OD), Abcam
Supplier: Abcam
20nm Gold conjugation / labeling in < 4 hrs with 30 secs hands-on time using Gold Conjugation Kit (20 nm, 20 OD) ab188215. 100% antibody recovery.
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FITC Conjugation Kit (Fast) - Lightning-Link®, Abcam
Supplier: Abcam
The Abcam FITC Conjugation Kit (ab188285) offers: -Speed and Efficiency: enables FITC conjugation or labeling in less than 20 minutes with only 30 seconds of hands-on time. -High Recovery: ensures 100% antibody recovery, meaning no loss of valuable antibodies. -Versatility: can be used to conjugate not only antibodies but also other proteins and peptides, making it suitable for various applications - Confidence: cited in over 15 publications
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Rhodamine Conjugation Kit (Fast) - Lightning-Link®, Abcam
Supplier: Abcam
Rhodamine conjugation / labeling in < 20 mins with 30 secs hands-on time using Rhodamine Conjugation Kit (Fast) - Lightning-Link® ab188286. 100% antibody recovery.
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Cy3® Conjugation Kit (Fast) - Lightning-Link®, Abcam
Supplier: Abcam
Cy3® conjugation / labeling in < 20 mins with 30 secs hands-on time using Cy3® Conjugation Kit (Fast) - Lightning-Link® ab188287. 100% antibody recovery.
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Gold-Maleimide Conjugation Kit (40nm, 20 OD), Abcam
Supplier: Abcam
40nm Gold conjugation / labeling in < 4 hrs with 30 secs hands-on time using Gold-Maleimide Conjugation Kit (40nm, 20 OD) ab188290. 100% antibody recovery.
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Streptavidin Gold Conjugate (40nm, 10 OD), Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using Streptavidin Gold Conjugate (40nm, 10 OD) ab186864. 100% antibody recovery.
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Biotin Gold Conjugate (40nm, 10 OD), Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using Biotin Gold Conjugate (40nm, 10 OD) ab186922. 100% antibody recovery.