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Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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rProtein A/G Beads 4FF, Abcam

Supplier: Abcam

Supplemental Information * Ligand: Recombinant Protein A/G * Basic Beads: 4% Cross-linked agarose beads * Particle size: 45μm – 165μm * Storage Buffer: 80% PBS, 20% ethanol * pH Stability Range: 3-10 * Maximum pressure: 0.3 MPa * Binding Capacity: 10-15 mg Rabbit lgG/ml .

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Organelle-ID™ RGB reagent II

Supplier: Enzo Life Sciences

Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids

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Alexa Fluor® 700 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Alexa Fluor® 700 conjugation / labeling in < 20 mins with 30 secs hands-on time using Alexa Fluor® 700 Conjugation Kit (Fast) - Lightning-Link® ab269824. 100% antibody recovery.

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Tyramide amplification buffer plus

Tyramide amplification buffer plus

Supplier: Biotium

An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

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R-Phycoerythrin (RPE)

R-Phycoerythrin (RPE)

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin.

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Bio-16-ddUTP, tetralithium salt

Bio-16-ddUTP, tetralithium salt

Supplier: Enzo Life Sciences

Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.

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Mouse and Rabbit Specific HRP/DAB Detection IHC kit, Abcam

Supplier: Abcam

HRP/DAB detection IHC kit, mouse & rabbit specific. Is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody. - Cited in over 220 publications - HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB

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Mouse Specific HRP/AEC Detection IHC kit, Abcam

Supplier: Abcam

Mouse specific HRP/AEC IHC Detection Kit ab64258 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.

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ExoBrite™ True EV Membrane Stains

Supplier: Biotium

Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.

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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Alexa Fluor® 488 protein labeling/conjugation kit, Lightning-Link® requires < 20 minutes with 30 seconds hands-on time. - Cited in over 30 publications - 100% antibody recovery - 15-minute incubation time Custom-size conjugation kits up to 100 mg are available on demand.

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Gold 550 dUTP

Supplier: Enzo Life Sciences

Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analogue can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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Gold 525 dUTP (lyophilised)

Supplier: Enzo Life Sciences

Gold 525 dUTP (lyophilised)

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Fixable Viability Dye (787/808), Abcam

Fixable Viability Dye (787/808), Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using Fixable Viability Dye (787/808) ab314683. 100% antibody recovery.

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Atto 488 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

ATTO 488 conjugation / labeling in < 20 mins with 30 secs hands-on time using Atto 488 Conjugation Kit (Fast) - Lightning-Link® ab269896. 100% antibody recovery.

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XFD750 Conjugation Kit (BSA compatible), Abcam

Supplier: Abcam

XFD750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using XFD750 Conjugation Kit (BSA compatible) ab312813. 100% antibody recovery.

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Streptavidin Atto 542 Monovalent Antibody Labeling Kit, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using Streptavidin Atto 542 Monovalent Antibody Labeling Kit ab272188. 100% antibody recovery.

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