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Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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HRP Antibody Conjugation Check Kit, Abcam

Supplier: Abcam

HRP conjugation / labeling in < 4 hrs with 30 secs hands-on time using HRP Antibody Conjugation Check Kit ab236555. 100% antibody recovery.

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Maxi fluorescent antibody labelling kits, Mix-n-Stain™, 1 mg labelling

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.

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Dye conjugates dUTP CF®

Supplier: Biotium

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

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Reactive quencher dyes

Supplier: Biotium

Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

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Green 496 dUTP

Supplier: Enzo Life Sciences

Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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ZnAF-2 DA

Supplier: Enzo Life Sciences

Fluorescent probe for Zn²⁺. Cell permeable.

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Alkaline phosphatase Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

Abcam's Alkaline Phosphatase Conjugation Kit (ab102850) offers : Rapid Conjugation: Achieve alkaline phosphatase labeling in under 4 hours with just 30 seconds of hands-on time. High Efficiency: Ensures 100% antibody recovery, preserving the integrity and value of your antibodies. Versatility: Suitable for conjugating antibodies, proteins, and peptides, and the labeled antibodies can be used immediately in applications such as Western Blotting (WB), ELISA, and Immunohistochemistry (IHC) without further purification.

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Streptavidin AZDye™ 594 Monovalent Antibody Labeling Kit, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using Streptavidin AZDye™ 594 Monovalent Antibody Labeling Kit ab272189. 100% antibody recovery.

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iFluor® Ultraviolet 350 Conjugation Kit, Abcam

Supplier: Abcam

iFluor™ 350 conjugation / labeling in < 4 hrs with 30 secs hands-on time using iFluor® Ultraviolet 350 Conjugation Kit ab312809. 100% antibody recovery.

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iFluor® Red 750 Conjugation Kit, Abcam

Supplier: Abcam

iFluor® Red 750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using iFluor® Red 750 Conjugation Kit ab312811. 100% antibody recovery.

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ExoBrite™ CTB EV Staining Kits, Biotium

Supplier: Biotium

ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ CTB EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.

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Biotin kits, Micro HOOK™

Supplier: G-Biosciences

The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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Bilirubin conjugate, sodium salt

Supplier: Cayman Chemical

Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.

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Alexa Fluor® 568 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Alexa Fluor® 568 conjugation / labeling in < 20 mins with 30 secs hands-on time using Alexa Fluor® 568 Conjugation Kit (Fast) - Lightning-Link® ab269821. 100% antibody recovery.

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Phosphoserine detection set

Supplier: Enzo Life Sciences

The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.

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TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & Green/HRP), Abcam

Supplier: Abcam

Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples.

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