Protein Extraction
Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.
EpiXtract® Nuclear protein isolation kit II (nucleic acid-free)
Supplier: Enzo Life Sciences
Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.
Expand 1 Items
EpiXtract® total histone extraction kit (high throughput)
Supplier: Enzo Life Sciences
Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.
Expand 1 Items
Protein fractionation kit, FOCUS™ signal proteins
Supplier: G-Biosciences
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
Expand 1 Items
FOCUS™ SubCell, protein fractionation kit
Supplier: G-Biosciences
FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.
Expand 1 Items
Protein reducing agents
Supplier: G-Biosciences
Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.
Expand 2 Items
Ingel™ for protein digestion
Supplier: G-Biosciences
InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
Expand 1 Items
Protein Extraction Kit, Abcam
Supplier: Abcam
Protein Extraction Kit (ab270054) is for the solubilization of proteins for a variety of proteomics applications.
Expand 1 Items
Protein quantitation kit, AccuOrange™
Supplier: Biotium
AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).
Expand 2 Items
Proteome kits, FOCUS™ Plant Proteome
Supplier: G-Biosciences
Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.
Expand 1 Items
Proteome kits, FOCUS™ Insect Proteome
Supplier: G-Biosciences
Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.
Expand 1 Items
Isobaric mass tagging kits and reagent sets, TMT™
Supplier: Thermo Fisher Scientific
Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.
Expand 8 Items
Thrombin cleavage kit, Abcam
Supplier: Abcam
Thrombin cleavage kit efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence. - Suitable for recombinant protein purification and tag removal - Contains sufficient active thrombin enzyme to cleave up to 5 mg of the target protein.
Expand 1 Items
Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer), Abcam
Supplier: Abcam
ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues.
Expand 1 Items
Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierce™
Supplier: Thermo Fisher Scientific
Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
Expand 1 Items
Proteome kits, FOCUS™ Bacterial Proteome
Supplier: G-Biosciences
Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from E.coli, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
Expand 1 Items
Albumin removal kit, AlbuminOUT™
Supplier: G-Biosciences
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.