Protein Extraction
Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.
Immobilised trypsin, TPCK treated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
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Mitochondria Isolation Kit for Tissue
Supplier: Thermo Fisher Scientific
The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.
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Detergent sampler Kit, Surfact-Amps™
Supplier: Thermo Fisher Scientific
Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.
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Phosphoprotein enrichment kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.
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Immobilised Trypsin
Supplier: G-Biosciences
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
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FOCUS™ Protein Alkylation Kit
Supplier: G-Biosciences
The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
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Carrier proteins, maleimide-activated carrier proteins, Imject™ Pierce™
Supplier: Thermo Fisher Scientific
Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.
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Trypsin protease, MS grade
Supplier: Thermo Fisher Scientific
Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
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Organelle Enrichment Kits
Supplier: Thermo Fisher Scientific
Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.
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Mitochondria isolation kit for cultured cells
Supplier: Thermo Fisher Scientific
The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.
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Protein concentration kit, Column-PROTEIN-Concentrate™
Supplier: G-Biosciences
The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.
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Tryptic Digest of Bovine Serum Albumin
Supplier: BRUKER DALTRONIK
Tryptic digest of bovine serum albumin, for 5×250 spots
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Protein precipitation and concentration kit, UPPA-PROTEIN-Concentrate™
Supplier: G-Biosciences
UPPA PROTEIN-Concentrate™ Kit uses a proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1 ng/ml. Concentration is not affected by the presence of common laboratory agents, including detergents and chaotropes. After precipitation, the sample is washed to remove salts and other interfering agents; complete recovery of sample is produced.
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Cell surface protein isolation and labelling kit, HOOK™
Supplier: G-Biosciences
The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.
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Protein concentration kit, OrgoSol-PROTEIN-Concentrate™
Supplier: G-Biosciences
The OrgoSol-PROTEIN-Concentration™ Kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the biological activity of most proteins.
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Carrier proteins, Imject™ Maleimide-Activated Blue Carrier™
Supplier: Thermo Fisher Scientific
Imject™ Maleimide-Activated Blue Carrier™ Protein enables conjugation of a sulfhydryl-containing hapten to elicit an immune response and antibody production against the hapten. Blue Carrier™ Protein is a haemocyanin from the Concholepas concholepas mollusc with immunogenic properties similar to keyhole limpet haemocyanin. Blue Carrier™ Protein has a large mass (~8 × 106 Da) and has been successfully used in polyclonal and monoclonal antibody production for
synthetic haptens and peptides.