Protein Extraction
Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.
Protein fractionation kit, nuclear and cytoplasmic extraction kit
Supplier: G-Biosciences
The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.
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Immobilized pepsin, Pierce™
Supplier: Thermo Fisher Scientific
Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
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Ubiquitin enrichment kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
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Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.
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Adenine deaminase
Supplier: G-Biosciences
Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
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FOCUS™ protein reductant kit
Supplier: G-Biosciences
FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
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FOCUS™ protein reduction-alkylation kit
Supplier: G-Biosciences
The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.
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Immobilised papain, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
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Isobaric mass tag labelling kit and reagent sets, TMT10plex™
Supplier: Thermo Fisher Scientific
TMT10plex™ Isobaric Mass Tag Labelling Kit and Reagent Sets enable multiplex relative quantitation by mass spectrometry (MS).
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Detergent removal spin column kit, HiPPR™
Supplier: Thermo Fisher Scientific
The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.
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Pierce™ Detergent Removal Spin Columns and Resin
Supplier: Thermo Fisher Scientific
Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.
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InGel™ Array kit for protein digestion
Supplier: G-Biosciences
InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.
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Cell surface protein isolation kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.
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Ovalbumin (in PBS), Imject™
Supplier: Thermo Fisher Scientific
Ovalbumin (in PBS), Imject™
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Immobilised Papain
Supplier: G-Biosciences
A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
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Micrococcal nuclease solution (≥100 U/µl)
Supplier: Thermo Fisher Scientific
This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.