Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer, HEPES solution, 1 M, suitable for cell culture, 0,2 μm filtered, BioPerformance Certified, Sigma-Aldrich®

Supplier: Merck

HEPES buffer solution (1 mol/l)

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pH buffers, for LAQUAtwin meters

Supplier: HORIBA

pH 7,00 buffer (6× 14 ml bottles per pack), Bottle

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), Sigma-Aldrich®

Supplier: Merck

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Buffer, TRIS solution pH 8,0 (2 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 8,0 (2 mol/l)

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Redox buffer solutions

Supplier: Mettler - Toledo

Redox buffer solutions are used for verification of all common redox sensors.

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Technical pH buffer solutions, HI 5000 series

Supplier: Hanna

pH Buffer solution, pH value: 7,01, ±0,01, Reference temperature: 25 °C, Plastic bottle

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Buffer, sodium acetate buffer solution pH 7 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, HEPES sodium salt solution (1 mol/L), BioReagent, suitable for cell culture, Sigma-Aldrich®

Supplier: Merck

HEPES sodium salt buffer solution (1 mol/l)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).