Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer tablets, Lovibond®

Supplier: Lovibond

Ideal for preparing buffer solutions of 4, 7 and 10 pH units.

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Buffer, TRIS solution pH 7,2 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,2 (1 mol/l)

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pH buffer solutions, pH 1,679

Supplier: Mettler - Toledo

pH buffer solution, pH value: 1,679

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Buffer, HEPES solution (1 mol/L), Sigma-Aldrich®

Supplier: Merck

HEPES buffer solution (1 mol/l)

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Phosphate buffered saline (PBS), Sigma-Aldrich®

Supplier: Merck

Contents of one pouch, when dissolved in one liter of distilled or deionised water, will yield 0,01 M phosphate buffered saline (NaCl 0,138 M; KCl - 0,0027 M); TWEEN® 20 - 0,05%, pH 7,4, at 25 °C.

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Triethylammonium bicarbonate buffers, for ion-exchange chromatography, Sigma-Aldrich®

Supplier: Merck

Buffer for use in ion-exchange chromatography and electrophoresis.

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pH Buffers

Supplier: Mettler - Toledo

pH buffer, 3,9 M NaCl, pH value: 7,00, 250 ml

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: SERACARE LIFE SCIENCES

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer, imidazole buffer solution (1 mol/L) BioUltra, Sigma-Aldrich®

Supplier: Merck

Imidazole buffer solution (1 mol/l)

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Glycine buffer, Sigma-Aldrich®

Supplier: Merck

For use in the enzymatic determination of lactate using lactic dehydrogenase.

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Technical pH buffer solutions in sachets, HI 50000 series

Supplier: Hanna

These technical calibration buffers produced in accordance with ISO 3696/BS3978 standards using high purity salts, deionised water, certified weight-checked balances, and Class A glassware in a temperature controlled environment monitored with certified thermometers.

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Dulbecco’s phosphate buffered saline (DPBS), Sigma-Aldrich®

Supplier: Merck

Dulbecco’s phosphate buffered saline is modified with/without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture.

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Buffer solutions

Supplier: Hanna

Solutions in sachets ensure optimum precision. The plastic-coated sachet provides a perfect liquid seal and good protection from light. The solutions should only be used once to avoid any danger of contamination. They also satisfy the requirements of the GLP and ISO standards. They have been adjusted using a pH meter that has been calibrated according to the NIST standards.