Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
GST buffer kit
Supplier: Cytiva
GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.
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Buffer Capsules
Supplier: Greisinger
Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.
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Tris-Glycine (TG) buffer,, MP Biomedicals
Supplier: MP Biomedicals
Tris-glycine buffer is used for western blotting and gel electrophoresis.
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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)
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Buffer solutions
Supplier: SI Analytics
Buffer solutions intended for extremely precise calibration.
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His buffer kit
Supplier: Cytiva
His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.
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Protein A/G HP SpinTrap™ Buffer Kit
Supplier: Cytiva
Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.
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Ab buffer kit
Supplier: Cytiva
Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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DIGE buffer kit
Supplier: Cytiva
DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.
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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals
Supplier: MP Biomedicals
Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.
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Buffer solutions as per DIN 19266-01: 2000
Supplier: WTW
These standard buffer solutions (work reference buffer solutions) are widely used in precision applications.
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pH buffers, ready to use
Supplier: WTW
Every bottle has its own lot reference with the expiration date.
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HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals
Supplier: MP Biomedicals
HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.
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Hank's balanced salt solution, modified, MP Biomedicals
Supplier: MP Biomedicals
Cell culture buffer, Hank's balanced salt solution, modified (1X)
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Low pH SDS Sample Buffer
Supplier: EKSIGENT
SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.