Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
TE buffer, pH 7,4, RNAse free
Supplier: Thermo Fisher Scientific
TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. It is used for storing and diluting DNA and RNA. Treatment with TE buffer enhances the staining intensity of antibodies in the immunohistochemical detection of certain proteins.
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TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100
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TRIS-buffered saline (TBS, 10X) pH 7,4
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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MES-SDS running buffer (20X)
Supplier: Thermo Fisher Scientific
Running Buffer, MES-SDS running buffer (20X)
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Kinase buffer II (5X)
Supplier: Thermo Fisher Scientific
Kinase buffer II (5X)
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Buffers, glycylglycine, 0,2 M solutions
Supplier: Thermo Fisher Scientific
Glycylglycine, buffer solution, 0,2 M, pH value: 8,5, Colourless, Odourless, Liquid
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TE buffer, pH 8,1, RNAse free
Supplier: Thermo Fisher Scientific
Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.
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Sodium bicarbonate, Corning®
Supplier: Corning
Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment
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Alkaline running buffer solution
Supplier: Thermo Fisher Scientific
Running Buffer, Alkaline running buffer solution
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Hybridisation buffers, formamide-free, HYB-LINK™
Supplier: G-Biosciences
Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.
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Cell lysis buffers
Supplier: Rockland Immunochemicals
Cell lysis buffer is used to lyse cells under non-denaturing conditions.
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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™
Supplier: HyClone products (Cytiva)
HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.
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MOPS-EDTA-sodium acetate buffer (MESA)
Supplier: Thermo Fisher Scientific
MOPS-EDTA-sodium acetate buffer (MESA), Liquid
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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®
Supplier: Corning
20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures