Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Phosphate-buffered saline (PBS, 10X), pH 7,4, for Western blot
Supplier: Thermo Fisher Scientific
Washing buffer, Phosphate-buffered saline (PBS, 10X)
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NP-40 lysis buffer, low salt
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, low salt, Thermo Fisher Scientific
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Stacking buffer (4X)
Supplier: Thermo Fisher Scientific
Electrophoresis, Stacking Buffer, 4X
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Phosphate, 0,2 M buffer solution, pH 7,6
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,2 M, pH value: 7,6, Liquid
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TRIS-glycine native sample buffer (4X), pH 8,6
Supplier: Thermo Fisher Scientific
Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)
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CHES, 0.5 M buffer solutions
Supplier: Thermo Fisher Scientific
2-(Cyclohexylamino)ethanesulfonic acid, CHES.
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Nitrocellulose stripping buffer (10X)
Supplier: Thermo Fisher Scientific
Electrophoresis, Nitrocellulose Stripping Buffer, 10X
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Buffer, ACES, 0,5 M solution
Supplier: Thermo Fisher Scientific
ACES, 0,5M buffer solution, pH 6,5, Colourless, Liquid
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MNE buffer, pH 6,5
Supplier: Thermo Fisher Scientific
MNE buffer, pH 6.5, Thermo Fisher Scientific
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TRIS-glycine-SDS running buffer (10X), pH 8,5
Supplier: Thermo Fisher Scientific
Running Buffer, TRIS-glycine-SDS running buffer (10X)
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TRIS-buffered saline (TBS, 10X) pH 7,6
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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HEPES-buffered saline, pH 7,0 (2X for transfection)
Supplier: Thermo Fisher Scientific
Cell culture buffer, HEPES-buffered Saline
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Phosphate, 0,5M buffer solution, pH 6,5
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,5 M, pH value: 6,5, Liquid
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HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific