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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Blocking buffers, FirstChoice™

Blocking buffers, FirstChoice™

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

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VWR®, pH electrode maintenance kit

VWR®, pH electrode maintenance kit

Supplier: VWR Chemicals

Buffers, electrolyte and cleaning solutions (100 ml), pH value: 4,00/7,00/10,00, Reference temperature: 20 °C, VWR®, Neutral, Plastic bottle

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J.T.Baker® DILUT-IT™, Buffer Solutions, Colour Coded, Concentrate

Supplier: Avantor

Volumetric concentrate, for dilution to 500 ml.

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Buffer, HEPES solution (1 mol/L)

Supplier: Cayman Chemical

HEPES buffer solution (1 mol/l)

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Phosphate Buffered Saline

Supplier: THERMO FISHER DIAGNOSTICS

Cell culture buffer, Phosphate buffered saline tablets

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Lithium nitrate, ionisation buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Lithium Nitrate Buffer Solution

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Wash buffer salts, lyophilised, Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.

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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.

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J.T.Baker®, Sheath fluid, 3200/3400

Supplier: Avantor

Sheath fluid, 3200/4000, J.T.Baker®

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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VWR® TISAB II Solution (Total Ionic Strength Adjustment Buffer) for Fluoride Determinations

VWR® TISAB II Solution (Total Ionic Strength Adjustment Buffer) for Fluoride Determinations

Supplier: VWR Chemicals

Total ionic strength adjustment buffer solution for use with selective ion electrodes.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: BIOMOL RESEARCH LABORATORIES

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Biotium

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TRIS solution pH 8 (1 mol/L)

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution (1 mol/l)

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J.T.Baker® DILUT-IT™, Sodium Phosphate, Dissolution Media Concentrate

J.T.Baker® DILUT-IT™, Sodium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT™ dissolution media concentrate, sodium phosphate, 961,5 ml, J.T.Baker® DILUT-IT®, pH value: 6,8

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Rockland Immunochemicals

EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.

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RNA isolation buffers

Supplier: OMEGA BIO-TEK

RNA Wash Buffer I

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MMP-1 assay buffer

Supplier: ENZO LIFE SCIENCES

MMP-1 assay buffer

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Buffer, triethylammonium acetate, 1 M solution, pH 7,0

Buffer, triethylammonium acetate, 1 M solution, pH 7,0

Supplier: PanReac AppliChem

Used for the purification of oligonucleotides.

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Hybridisation buffers

Hybridisation buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

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Gram Negative lysis buffer

Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Fixation, (buffer)

Supplier: ENZO LIFE SCIENCES

Fixation, (buffer)

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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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2X SDS-PAGE sample buffer without DTT or b-ME

Supplier: Rockland Immunochemicals

Sample buffer for protein electrophoresis.

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Mini flip-top vial with 9 ml Butterfield's buffer

Mini flip-top vial with 9 ml Butterfield's buffer

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Butterfield's Buffer is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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Proteasome assay buffer

Supplier: ENZO LIFE SCIENCES

Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.

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HDAC assay buffer

Supplier: ENZO LIFE SCIENCES

HDAC assay buffer

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