Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Non-animal sera protein blocking Agent, FISH-Blocker™
Supplier: G-Biosciences
FISH-Blocker™ uses a fish protein as the primary blocking agent to eliminate cross reactivity. Using a non mammalian protein eliminates or minimises the interaction of antibodies raised in mammals. It is offered either in TBS (Tris-buffered saline) or in PBS (phosphate-buffered saline) at pH 7,5.
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Protein extraction kit, Insect PE LB™
Supplier: G-Biosciences
Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Western Blot Transfer buffer, 10X, methanol-free, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Methanol-Free 10X Western Blot Transfer Buffer is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.
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Subcellular protein fractionation kit for tissues
Supplier: Thermo Fisher Scientific
This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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pH buffer solutions
Supplier: Hach
pH buffer standard solution with certificate of analysis, 125 ml, pH value: 9,21, Plastic bottle
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Luciferase cell lysis buffer (2X), Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
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Pierce TMT™ Elution Buffer
Supplier: Thermo Fisher Scientific
Thermo Scientific TMT Elution Buffer is for use with Thermo Scientific Immobilized Anti-TMT Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.
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Wash buffer, Pierce™ Fast Wash Buffer
Supplier: Thermo Fisher Scientific
Pierce™ Fast Wash buffer, 10X, for use with Pierce™ Fast Blocking buffer.
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Blocking buffer, fast blocking, Pierce™
Supplier: Thermo Fisher Scientific
This buffer effectively and reliably blocks Western blots in just five minutes to provide low background results and speed up traditional blotting protocols.
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Blocking buffer, concentrate, Pierce™ Clear Milk
Supplier: Thermo Fisher Scientific
This clear milk blocking buffer is a long shelf life, high performance replacement for homemade milk blocking buffers in Western blotting applications.
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Dulbecco's phosphate-buffered saline (DPBS), Corning®
Supplier: Corning
Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7,0 to 7,6
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Phosphate-Buffered saline (PBS), 10X concentrate, pH 7,4, molecular biology grade, Corning®
Supplier: Corning
Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research
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Hank's balanced salt solutions (HBSS), Corning®
Supplier: Corning
Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.
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Phosphate-buffered saline (PBS), Corning®
Supplier: Corning
Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate
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Buffer solutions, pH 4,00
Supplier: PanReac AppliChem
Buffer solutions contain citric acid 1 H₂O (11,768 g); sodium hydroxide 50% (6 ml); hydrochlorid acid 35% (3,96 ml); preservative 1% (5 ml) and water (1 l).