Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
HyClone™ Earle's 1X Balanced Salt Solution (EBSS): Solution
Supplier: HyClone products (Cytiva)
Balanced salt solution for cell culture applications.
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Dulbecco's phosphate buffered saline (DPBS), ES cell qualified, HyClone™ AdvanceSTEM™
Supplier: HyClone products (Cytiva)
A balanced salt solution tested for use with embryonic stem cells.
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Wash buffer, Pierce™
Supplier: Thermo Fisher Scientific
These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.
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Pierce™ Buffer, PBS Solution 20X Concentrate (Phosphate Buffered Saline)
Supplier: Thermo Fisher Scientific
Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.
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TAE buffer (20X), Pierce™
Supplier: Thermo Fisher Scientific
20X TAE buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Acetate EDTA running buffer used for agarose gel electrophoresis of nucleic acids.
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Pierce™ Buffer, TBS Solution 20X Concentrate (TRIS Buffered Saline)
Supplier: Thermo Fisher Scientific
Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.
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Electrophoresis running buffers, Pierce™
Supplier: Thermo Fisher Scientific
These are space-saving stock solution that is ideal for quickly preparing running buffer used for gel electrophoresis.
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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Pierce™
Supplier: Thermo Fisher Scientific
Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.
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I-PER™ Insect Cell Protein Extraction Reagents
Supplier: Thermo Fisher Scientific
The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
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TAE buffer, 50X
Supplier: EDVOTEK
This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.
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Tris-Glycine buffer
Supplier: EDVOTEK
For vertical polyacrylamide gel electrophoresis.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: OMEGA BIO-TEK
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Subcellular Protein Fractionation Kit for Cultured Cells
Supplier: Thermo Fisher Scientific
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
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IP lysis buffer, Pierce™
Supplier: Thermo Fisher Scientific
The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.
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Buffer, TE Solution (TRIS-EDTA buffer) pH 8,0, Low EDTA for Molecular Biology
Supplier: PanReac AppliChem
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0