Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Research buffers and reagents
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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Wash buffers, femto TBST™ and femto PBST™
Supplier: G-Biosciences
The femto TBST™ and femto PBST™ wash buffers will enhance sensitivity of immunoassays by minimising the 'washing out' of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resulting in a larger ratio of signal to background. These can be used for incubation and washing procedures.
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Protein extraction kit, bacterial PE LB™
Supplier: G-Biosciences
Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.
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Protein extraction kit, yeast PE LB™
Supplier: G-Biosciences
Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.
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Protein extraction kit, mammalian cell PE LB™
Supplier: G-Biosciences
Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
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Protein extraction kit, tissue PE LB™
Supplier: G-Biosciences
Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.
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Inclusion bodies solubilisation buffer
Supplier: G-Biosciences
The IBS™ buffer is specifically developed for solubilisation of inclusion bodies.
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Protein blocking solution, BLOK™ BLOTTO
Supplier: G-Biosciences
BLOK™ BLOTTO is a 5% novel modified milk protein solution used for Westerns, ELISA and dot blot blocking.
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Blocking solution, BLOK™ BSA
Supplier: G-Biosciences
BLOK™ BSA is a 10% BSA protein blocking solution.
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Blocking solution, BLOK™ Casein
Supplier: G-Biosciences
BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.
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Extraction buffers, FOCUS™
Supplier: G-Biosciences
FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.
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Equilibration buffer for IPG strips
Supplier: G-Biosciences
Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.
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DNA/RNA clean-up buffers
Supplier: OMEGA BIO-TEK
Binding buffer XP2, Omega Bio-Tek
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Phosphate buffered saline solutions (PBS) without calcium and magnesium, HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solution used for multiple cell culture applications.
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Pierce™ Blocking Buffers, Protein Free
Supplier: Thermo Fisher Scientific
These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.
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RIPA lysis and extraction buffer, Pierce™
Supplier: Thermo Fisher Scientific
The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.