Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Equilibration buffer for IPG strips
Supplier: G-Biosciences
Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.
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NucleoSpin® Kits for Genomic DNA Extraction
Supplier: MACHEREY-NAGEL
Combining selective purification technologies and chemistries, these are a comprehensive range of specialised DNA isolation kits for a broad range of diversified starting materials. The resulting high quality DNA serves as excellent basis for following demanding downstream applications.
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer) pH 8,3
Supplier: PanReac AppliChem
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer) pH 8,3
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Inclusion bodies solubilisation buffer
Supplier: G-Biosciences
The IBS™ buffer is specifically developed for solubilisation of inclusion bodies.
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Protein extraction kit, mammalian cell PE LB™
Supplier: G-Biosciences
Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
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Wash buffers, femto TBST™ and femto PBST™
Supplier: G-Biosciences
The femto TBST™ and femto PBST™ wash buffers will enhance sensitivity of immunoassays by minimising the 'washing out' of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resulting in a larger ratio of signal to background. These can be used for incubation and washing procedures.
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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer
Supplier: Thermo Fisher Scientific
Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.
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ReadiUse™ Viral RNA Lysis Buffer
Supplier: AAT Bioquest
Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.
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Pierce™ Blocking Buffers, Protein Free
Supplier: Thermo Fisher Scientific
These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.
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pH buffer solutions
Supplier: Hach
pH buffer standard solution with certificate of analysis, 125 ml, pH value: 7,00, Plastic bottle
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Subcellular Protein Fractionation Kit for Cultured Cells
Supplier: Thermo Fisher Scientific
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
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TAE buffer, 50X
Supplier: EDVOTEK
This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.
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Gel loading solution, 10X
Supplier: EDVOTEK
1 ml concentrate for 200 samples.
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VHB DNA Washing Buffer
Supplier: OMEGA BIO-TEK
VHB buffer must be diluted with ethanol before use.
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ReadiUse™ Cell Detaching Buffer (50 ml)
Supplier: AAT Bioquest
ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products.
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Protein extraction kit, bacterial PE LB™
Supplier: G-Biosciences
Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.