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Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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North2South™ Hybridisation Buffer

North2South™ Hybridisation Buffer

Supplier: Thermo Fisher Scientific

North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.

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Substrate buffers, Pierce™

Substrate buffers, Pierce™

Supplier: Thermo Fisher Scientific

These stable peroxide and diethanolamine substrate buffers produce optimised, reliable and convenient stock solutions for HRP and alkaline phosphatase assays detection respectively.

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ReadiUse™ Mammalian Cell Lysis Buffer *5X*

ReadiUse™ Mammalian Cell Lysis Buffer *5X*

Supplier: AAT Bioquest

All our ReadiUse™ reagents require minimal hands-on time.

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pH buffer, single-use, SINGLET™

pH buffer, single-use, SINGLET™

Supplier: Hach

These buffers solutions packs are used for pH meter calibration. The sealed foil pouches can be transported easily and safely without any spillage and also prevents contamination or exposure to air of the solutions.

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pH buffers, DuraCal

pH buffers, DuraCal

Supplier: HAMILTON BONADUZ

For full information concerning health and safety data please contact Avantor or Hamilton directly.

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Mammalian protein extract reagents, M-PER™

Mammalian protein extract reagents, M-PER™

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

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Phosphate-buffered saline (PBS), Corning®

Phosphate-buffered saline (PBS), Corning®

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

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Binding and elution buffers for antibody purification, Pierce™

Binding and elution buffers for antibody purification, Pierce™

Supplier: Thermo Fisher Scientific

These buffers ensure high-yield, non denaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

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Blocking buffers, protein-free, Pierce™

Blocking buffers, protein-free, Pierce™

Supplier: Thermo Fisher Scientific

Pierce Protein-free blocking buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

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Extraction buffers, FOCUS™

Extraction buffers, FOCUS™

Supplier: G-Biosciences

FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

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TE Buffers 100X

Supplier: G-Biosciences

TE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

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Blocking buffer, dry blend, SuperBlock™

Supplier: Thermo Fisher Scientific

SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

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Nuclear and cytoplasmic extraction reagents, NE-PER

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

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Genomic DNA extraction buffers

Supplier: OMEGA BIO-TEK

P2 buffer, Omega Bio-Tek

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Tris-Acetate-EDTA (TAE) buffer, 10X, molecular biology grade

Supplier: PanReac AppliChem

10X concentrated solution of 40 mM Tris, 20 mM glacial acetic acid and 1 mM EDTA.Na₂, pH 8,5 ±0,2.

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ReadiUse™ Universal Kinase Reaction Stopping Buffer

ReadiUse™ Universal Kinase Reaction Stopping Buffer

Supplier: AAT Bioquest

ReadiUse™ Universal Kinase Reaction Stopping Buffer is an aqueous solution that effectively chelates divalent metal ions.

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