Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Blocking buffer, fish gelatin
Supplier: Biotium
Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
Expand 2 Items
RIPA cell lysis buffer 2
Supplier: Enzo Life Sciences
RIPA cell lysis buffer 2, Enzo Life Sciences
Expand 1 Items
100x Citrate Buffer pH 6.0, Abcam
Supplier: Abcam
Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC (ab64236).
Expand 1 Items
Caspase assay buffer
Supplier: Enzo Life Sciences
50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.
Expand 1 Items
Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)
Supplier: Rockland Immunochemicals
Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Expand 6 Items
Buffer, TE Solution (TRIS-EDTA buffer) pH 8,0, Low EDTA for Molecular Biology
Supplier: PanReac AppliChem
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0
Expand 2 Items
Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: OMEGA BIO-TEK
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
Expand 1 Items
Wash buffer, Pierce™
Supplier: Thermo Fisher Scientific
These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.
Expand 5 Items
Hank's balanced salt solutions (HBSS), Corning®
Supplier: Corning
Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.
Expand 9 Items
Pierce™ BupH™ Wash Buffers, Dry Blend Pack
Supplier: Thermo Fisher Scientific
BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
Expand 8 Items
Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable
Supplier: Thermo Fisher Scientific
This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
Expand 1 Items
Blocking solution, BLOK™ Casein
Supplier: G-Biosciences
BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.
Expand 2 Items
ReadiUse™ Mammalian Cell Lysis Buffer *5X*
Supplier: AAT Bioquest
All our ReadiUse™ reagents require minimal hands-on time.
Expand 1 Items
pH buffer, single-use, SINGLET™
Supplier: Hach
These buffers solutions packs are used for pH meter calibration. The sealed foil pouches can be transported easily and safely without any spillage and also prevents contamination or exposure to air of the solutions.