Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, HEPES solution (1 mol/L)
Supplier: Cayman Chemical
HEPES buffer solution (1 mol/l)
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Plasmid DNA buffers
Supplier: OMEGA BIO-TEK
DNA wash buffer concentrate, low salt spin column was solution (makes 250 ml)
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Background suppressor system (Permeabilising), TrueBlack® IF
Supplier: Biotium
A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
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Proteometer-CV consumables kit
Supplier: NOVILYTIC
The Proteometer-CV is designed for the scientific development of mAbs, bi-specifics, or fusion proteins with a human Fc region, regardless of their stage in the development continuum. This kit offers an alternative to traditional charge variant methods that require Protein A.
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E.Z.N.A.® RNALock stabilizer reagent
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.
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Proteometer-L fluorescent reagent
Supplier: NOVILYTIC
7 vials of the Proteometer-L fluorescent reagent specifically formulated for use with Proteometer-L kit.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation
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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0), Abcam
Supplier: Abcam
Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: Thermo Fisher Scientific
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Competent cell buffer
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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2X SDS-PAGE sample buffer without DTT or b-ME
Supplier: Rockland Immunochemicals
Sample buffer for protein electrophoresis.
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Buffer, TRIS solution pH 8 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)
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Lithium nitrate, ionisation buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Lithium Nitrate Buffer Solution
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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Electrophoresis running buffers
Supplier: G-Biosciences
There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.



