Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Fixation buffer for microscopy and flow cytometry
Supplier: Biotium
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.
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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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HEPES Buffer pH 8.0 (1 M), Sterile
Supplier: Apollo Scientific
Aqueous solution
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G-Biosciences TAE Running Buffer (50X)
Supplier: G-Biosciences
The primary use of TAE Running Buffer is in agarose electrophoresis for the separation of nucleic acids (DNA and RNA).
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TES buffer, 1X, ready to use, pH 7,8
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1,0 mM EDTA at a pH of 7,8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionised water.
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Proteasome assay buffer
Supplier: Enzo Life Sciences
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: Enzo Life Sciences
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
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20x TBS-T with Tween 20, Abcam
Supplier: Abcam
Tris Buffered Saline (TBS) wash buffer with Tween 20 for use during immunolabeling techniques such as IHC.
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Diluent buffer, 10X concentrate, for dab membrane substrate
Supplier: Rockland Immunochemicals
Concentrate diluent buffer for DAB membrane substrate, 10X
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Buffer solution pH 10, carbonate buffer
Supplier: Thermo Fisher Scientific
CAS No.: 1332-57-6
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HEPES Buffer Solution 1 M, HyClone™
Supplier: HyClone products (Cytiva)
Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.
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Cell culture salts, salt solutions and buffers
Supplier: Biowest
These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.
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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0), Abcam
Supplier: Abcam
Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Background suppressor system (Permeabilising), TrueBlack® IF
Supplier: Biotium
A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.