Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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TPE buffer, 10X tris-phosphate-EDTA, pH 8
Supplier: Rockland Immunochemicals
Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)
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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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SDS-Page running gel buffers, 10X concentrate
Supplier: Rockland Immunochemicals
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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2X SDS-PAGE sample buffer without DTT or b-ME
Supplier: Rockland Immunochemicals
Sample buffer for protein electrophoresis.
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: Rockland Immunochemicals
This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
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SSC buffer, 20X concentrate, pH 7,0
Supplier: Rockland Immunochemicals
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: Rockland Immunochemicals
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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Buffers, TE solutions 10X concentrates (TRIS-EDTA buffer)
Supplier: Rockland Immunochemicals
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
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Buffer, TE solution (TRIS-EDTA buffer) pH 8,0
Supplier: Rockland Immunochemicals
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0
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TES buffer, 1X, ready to use, pH 7,8
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1,0 mM EDTA at a pH of 7,8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionised water.
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Buffer HF-Improved
Supplier: Thermo Fisher Scientific
Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.
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Dry buffer packs, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.
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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.