Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Tris-Borate-EDTA (TBE) buffer, 10X
Supplier: PanReac AppliChem
10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.
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Buffer, triethylammonium acetate, 1 M solution, pH 7,0
Supplier: PanReac AppliChem
Used for the purification of oligonucleotides.
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: PanReac AppliChem
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Buffer, TRIS solution pH 8 (1 mol/L)
Supplier: PanReac AppliChem
Cloning Buffer, TRIS buffer solution (1 mol/l)
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Buffer kit for calibration of fluorescent Ca² indicators
Supplier: Biotium
Kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca² indicators (1,2).
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: Thermo Fisher Scientific
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Cell culture salts, salt solutions and buffers
Supplier: Biowest
These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.
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TM Buffer, 1X Tris-Magnesium sulphate, pH 7,5
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 50 mM Tris HCl, 10 mM magnesium sulphate at pH 7,5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionised water.
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Diluent buffer, 10X concentrate, for dab membrane substrate
Supplier: Rockland Immunochemicals
Concentrate diluent buffer for DAB membrane substrate, 10X
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Competent cell buffer
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
Supplier: Rockland Immunochemicals
EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.
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GET buffer
Supplier: Rockland Immunochemicals
GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.
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PBS and TBS buffers
Supplier: Rockland Immunochemicals
Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.
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Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)
Supplier: Rockland Immunochemicals
Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.