Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
25x PBS Buffer pH 7.6, Abcam
Supplier: Abcam
In order to provide this product at 25X, the salts in solution are at a very high concentration.
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10x Citrate Buffer pH 6.0, Abcam
Supplier: Abcam
Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC.
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10x EDTA Buffer pH 8.0, Abcam
Supplier: Abcam
EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).
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Protein Block, Abcam
Supplier: Abcam
Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required
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100x Citrate Buffer pH 6.0, Abcam
Supplier: Abcam
Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC (ab64236).
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Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0), Abcam
Supplier: Abcam
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0), Abcam
Supplier: Abcam
Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
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Blocking buffer, fish gelatin
Supplier: Biotium
Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
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G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)
Supplier: G-Biosciences
The tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.
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PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Biotium
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Gram Negative lysis buffer
Supplier: G-Biosciences
Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.
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Buffer, PBS tablets
Supplier: PanReac AppliChem
PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation
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Nuclear (Nucleic Acid-Free) Extraction Kit, Abcam
Supplier: Abcam
Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.
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Buffer, TRIS solution pH 7 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)