Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
HEPES Buffer Solution 1 M, HyClone™
Supplier: HyClone products (Cytiva)
Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.
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Cesium nitrate, ionization buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Cesium Nitrate Buffer solution
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Lithium nitrate, ionisation buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Lithium Nitrate Buffer Solution
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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solution used for multiple cell culture applications (powder form).
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Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solutions for cell culture applications.
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Dulbecco's phosphate buffered saline (DPBS), for cell culture, HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solution used for multiple cell culture applications, (liquid form).
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Blocking buffers, Protein-Free™
Supplier: G-Biosciences
Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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G-Biosciences TBE-Urea Sample Buffer
Supplier: G-Biosciences
Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands.
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20x TBS-T with Tween 20, Abcam
Supplier: Abcam
Tris Buffered Saline (TBS) wash buffer with Tween 20 for use during immunolabeling techniques such as IHC.
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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0), Abcam
Supplier: Abcam
Antigen retrieval 100X tris-EDTA buffer (pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. - Cited in over 20 publications - Contains 5% tris, 5% tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol - Optimized for tris-EDTA buffer (pH 9.0) treated antibodies - Contains detergent for paraffin emulsification
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Antigen Retrieval Buffer (100X Citrate Buffer), Abcam
Supplier: Abcam
Antigen retrieval 100X citrate buffer (pH 5.1 - 5.5) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. - Cited in over 25 publications - Contains citrate buffer, 1.5% 2-butoxyethanol, 1% hydrochloric acid - Optimized for citrate buffer-treated antibodies - Contains detergent for paraffin emulsification
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: Thermo Fisher Scientific
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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25x PBS Buffer pH 7.6, Abcam
Supplier: Abcam
In order to provide this product at 25X, the salts in solution are at a very high concentration.



