An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.
Epigenetics Screening Library
Supplier: Cayman Chemical
For screening a variety of epigenetic compounds.
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FLUOR DE LYS® HDAC8 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
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ADAM17 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.
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LEADING LIGHT® Wnt cell line medium pack
Supplier: ENZO LIFE SCIENCES
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
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Black IHC chromogen (HRP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.
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Micro Bicinchoninic Acid (BCA) protein assay
Supplier: G-Biosciences
The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).
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Myeloperoxidase fluorometric detection kit
Supplier: ENZO LIFE SCIENCES
The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.
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Solutions colorantes, Ghost Dye™
Supplier: Tonbo Biosciences
Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.
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Protease Estimation Assay Kit, ProteSEEKER™
Supplier: G-Biosciences
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay. ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.
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Free Fatty Acid Fluorometric Assay Kit
Supplier: Cayman Chemical
A sensitive tool for measuring free fatty acids in plasma, serum, and urine.
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Matrix Metalloproteinase-7 (MMP-7) colorimetric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay SystemThe MMP-7 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a thiopeptide as a chromogenic substrate.
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PKC kinase activity kit
Supplier: ENZO LIFE SCIENCES
The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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CFDA SE Cell proliferation assay kit
Supplier: Biotium
Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.
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Red long-term cell tracer, CYTO-ID®
Supplier: ENZO LIFE SCIENCES
CYTO-ID® is a live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.
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CV-Caspase 3 & 7 Detection kit
Supplier: ENZO LIFE SCIENCES
Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.
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Apoptosis inducer set
Supplier: G-Biosciences
Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.
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Matrix Metalloproteinase-9 (MMP-9) colorimetric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-9 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a thiopeptide as a chromogenic substrate.
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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium
Supplier: Biotium
RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.
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Cellular senescence activity assay
Supplier: ENZO LIFE SCIENCES
Highly reproducible SA-β-gal activity kit
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Compatible Lowry protein assay
Supplier: G-Biosciences
These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.
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Flow cytometry fixation/permeabilisation kit
Supplier: Biotium
Flow Cytometry Fixation/Permeabilisation Kit contains optimally formulated buffers for fixation and permeabilisation of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. The bufffers are also available separately.
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Matrix Metalloproteinase-1 (MMP-1) colorimetric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. The MMP-1 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-1 (MMP-1, interstitial collagenase, fibroblast collagenase) using a thiopeptide as a chromogenic substrate.
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IHC diluent (primary Ab)
Supplier: ENZO LIFE SCIENCES
Immunohistochemistry diluent (primary Ab) can be used as a diluent to prepare primary antibodies and negative controls.
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Millicell® µ-Migration Assay Kit
Supplier: Merck
This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours.
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IHC enhancer (DAB, plus), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
HIGHDEF® IHC enhancer (DAB, plus) is a DAB signal enhancer used to increase intensity of immunostaining using DAB as chromogen.
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Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.
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Annexin V CF® dye conjugates, azide-free, lyophilised
Supplier: Biotium
Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments, fluorescence microscopy, or flow cytometry.
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MMP Inhibitor profiling kit, fluorometric
Supplier: ENZO LIFE SCIENCES
A QUANTIZYME® Assay System Complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes. The assays are performed in a convenient 96-well microplate format using a sensitive fluorogenic substrate . Included are active MMP enzymes, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.
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Blue IHC chromogen (AP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
Blue IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.
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ROS-ID™ total ROS/superoxide detection kit
Supplier: ENZO LIFE SCIENCES
The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.