You searched for: Assays

Supplier: Abcam

Simple, 1-step assay for screening potential α-amylase inhibitors.

Supplier: Thermo Fisher Scientific

The Compat-Able™ Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 min with just a few simple steps. The Compat-Able™ Preparation Set is available conveniently bundled with the Pierce BCA™ Protein Assay Kit.

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Supplier: Thermo Fisher Scientific

The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Supplier: G-Biosciences

The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

Supplier: Cayman Chemical

A sensitive tool for measuring free fatty acids in plasma, serum, and urine.

Supplier: G-Biosciences

CytoScan™ WST-1 Cell Proliferation Assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow coloured formazan is measured at 420 to 480 nm (optimal at 440 nm) and is directly correlated to cell number.

Supplier: Enzo Life Sciences

Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Supplier: Thermo Fisher Scientific

The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

Supplier: OZ BIOSCIENCES

BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content, even in the presence of detergents.

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

Supplier: Enzo Life Sciences

A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: Enzo Life Sciences

Hydrogen peroxide colorimetric detection kit provides a fast and higher throughput 96-well plate based homogeneous assay for the measurement of hydrogen peroxide in a wide variety of samples.

Supplier: OZ BIOSCIENCES

Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. This ready to use kit is provided with 1X reagent and 2 sets of BSA standards which means no dilution, filtration or calculation required. B-Pak is provided with adapted protocols for all experimental conditions – micro and macroassays.

Supplier: AAT Bioquest

The kit does not require cell lysis, long incubations or removal of cells with minimal hands-on time. It is compatible with high throughput screening.

Supplier: Abcam

cAMP Assay Kit (Competitive ELISA) is a Competitive ELISA kit for the measurement of cAMP Assay Kit (Competitive ELISA) in Mammals in Tissue Lysate, Saliva, Cell culture media, Cell culture supernatant, Serum, Cell Lysate samples.

Supplier: AAT Bioquest

Cell Meter™ flow cytometric calcium assay kit provides fluorescence-based assays for detecting intracellular calcium mobilisation using a flow cytometer.

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

Supplier: Enzo Life Sciences

The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.

Supplier: AAT Bioquest

Ready-to-use buffer that is optimised for calcium assays.