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Assays

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

Bicinchoninic acid disodium salt hydrate

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

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VWR®, Bradford protein assay, proteomics grade

VWR®, Bradford protein assay, proteomics grade

Supplier: VWR Chemicals

The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.

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VWR® Protein Precipitation Kit

VWR® Protein Precipitation Kit

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

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Cell-based assay calcein AM

Supplier: Cayman Chemical

Cellular assay, Cell-based assay calcein AM

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Triglyceride colorimetric assay kit

Supplier: Cayman Chemical

Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.

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Phagocytosis assay kit (IgG FITC)

Supplier: Cayman Chemical

Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.

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Proteasome 20S Assay Kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome.

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ATP-Glo™ Bioluminometric cell viability assay

ATP-Glo™ Bioluminometric cell viability assay

Supplier: Biotium

ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.

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Cholesterol cell-based detection assay kit

Supplier: Cayman Chemical

Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

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LDH Cytotoxicity WST Assay

Supplier: ENZO LIFE SCIENCES

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.

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NF-κB (p65) transcription factor assay kit

NF-κB (p65) transcription factor assay kit

Supplier: Cayman Chemical

NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).

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OXI-TEK TBARS assay kit

OXI-TEK TBARS assay kit

Supplier: ENZO LIFE SCIENCES

The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.

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SREBP-1 transcription factor assay kit

SREBP-1 transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of SREBP-1.

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LEADING LIGHT® Wnt Reporter Assay Starter Kit

Supplier: ENZO LIFE SCIENCES

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

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Calcium assay kits, FLIPR® Calcium 5 assay kit

Calcium assay kits, FLIPR® Calcium 5 assay kit

Supplier: Molecular Devices

The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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Superoxide dismutase assay kit

Superoxide dismutase assay kit

Supplier: Cayman Chemical

Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.

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Firefly luciferase assay kit (Lyophilised)

Supplier: Biotium

This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

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Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Supplier: G-Biosciences

A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.

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eFluxx-ID® multidrug resistance assay kit

Supplier: ENZO LIFE SCIENCES

The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells

Supplier: Biotium

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

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VWR® Fluorescent DNA Quantitation Kit

VWR® Fluorescent DNA Quantitation Kit

Supplier: VWR Chemicals

Fluorescent DNA Quantitation Kit uses fluorescent bisBenzimide H33258 dye to bind to AT sequences of the minor groove of double-stranded DNA. In the assay, the sample is excited at 360 nm and when the dye binds to dsDNA an emission spectrum at 460 nm is produced.

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Glycolysis Cell-Based Assay Kit

Glycolysis Cell-Based Assay Kit

Supplier: Cayman Chemical

A colorimetric-based method for detecting L-lactate in culture medium.

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QBT™ Fatty acid uptake assay kit

QBT™ Fatty acid uptake assay kit

Supplier: Molecular Devices

QBT™ Fatty Acid Uptake Assay Kit is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.

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Protein assay kits, RED 660™

Protein assay kits, RED 660™

Supplier: G-Biosciences

RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

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Thiol Detection Assay Kits

Thiol Detection Assay Kits

Supplier: Cayman Chemical

A simple, reproducible, and sensitive tool for assaying free thiol content in samples.

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Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit

Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit

Supplier: Molecular Devices

SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.

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LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit

Supplier: Cayman Chemical

For measurement of LDH activity as an indicator of cell death.

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Protein carbonyl colorimetric assay kit

Supplier: Cayman Chemical

The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.

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ProteoStat™ thioredoxin-1 assay kit for microplates

Supplier: ENZO LIFE SCIENCES

This kit provides a simple, homogenous assay for screening modulators of Thioredoxin-1 enzymatic activity.

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Calcineurin cellular activity assay kit

Calcineurin cellular activity assay kit

Supplier: ENZO LIFE SCIENCES

A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.

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