End-point PCR Enzymes and Kits
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
VWR®, TEMPase Hot Start 2× master mix
Supplier: VWR Chemicals
TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.
Expand 5 Items
VWR®, Red Taq DNA polymerase
Supplier: VWR Chemicals
Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.
Expand 4 Items
VWR® Fast HiFi Hot Start DNA Polymerase
Supplier: VWR Chemicals
Fast HiFi Hot Start DNA Polymerase is a thermostable, chimeric DNA polymerase created specifically for low-bias, high fidelity amplification of a vast range of amplicons.
Expand 4 Items
VWR®, Taq DNA polymerase, 500 U
Supplier: VWR Chemicals
Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of Taq DNA Polymerase to a 25 µl PCR reaction.
Expand 1 Items
VWR®, Taq DNA polymerase, 200000 U
Supplier: VWR Chemicals
Taq DNA polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kDa.
Expand 1 Items
VWR®, TEMPase Hot Start DNA polymerase
Supplier: VWR Chemicals
VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR. A glycerol-free TEMPase Hot Start DNA Polymerase is also available for automation and freeze drying.
Expand 7 Items
VWR®, PCR buffers
Supplier: VWR Chemicals
An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial.
Expand 3 Items
VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix
Supplier: VWR Chemicals
VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.
Expand 4 Items
VWR®, Taq DNA Polymerase, Glycerol-Free
Supplier: VWR Chemicals
Highly concentrated Taq DNA polymerase glycerol free 50 U/µl is ideal for industrial use and manufactures of diagnostic PCR kits.
Expand 3 Items
VWR® Fast HiFi Hot Start DNA Polymerase 2X Master Mix
Supplier: VWR Chemicals
Fast HiFi Hot Start DNA Polymerase 2X Master Mix is a convenient alternative to Fast HiFi Hot Start DNA Polymerase, displaying the same set of features. Fast HiFi Hot Start DNA Polymerase 2X Master Mix is also available with a red dye for direct gel loading.
Expand 4 Items
TriLink® Engineered RNase Inhibitor
Supplier: TriLink BioTechnologies
Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.
Expand 2 Items
AMPIGENE® Taq mix
Supplier: Enzo Life Sciences
Optimised standard PCR mix for enhanced speed, yield, and specificity. Used for standard PCR applications.
Expand 3 Items
Taq DNA polymerase, 1000 U
Supplier: Genscript
Taq DNA polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene.
Expand 1 Items
PerfeCTa® PreAmp 5X SuperMix
Supplier: Quantabio
PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.
Expand 1 Items
AccuStart™ master mixes, long range SuperMix
Supplier: Quantabio
Superior sensitivity and multiplexing for DNA amplification of long targets.
Expand 2 Items
AccuStart™ II PCR ToughMix®
Supplier: Quantabio
AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.