Urea
Urea ≥95%
Supplier: Apollo Scientific
Usage: Biochemical/life science applications.
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Urea 98%, pearls, extra pure
Supplier: Thermo Fisher Scientific
Urea 98%, pearls, Extra Pure
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Urea 99.5% for analysis
Supplier: Thermo Fisher Scientific
Urea 99.5% for analysis
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Urea 99.0-100.5% ACS
Supplier: Thermo Fisher Scientific
Urea 99.0-100.5% ACS
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Urea 99.5% for molecular biology DNase-, RNase-, protease-free
Supplier: Thermo Fisher Scientific
Urea 99.5% for molecular biology DNase-, RNase-, protease-free
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Urea 99.5-100.5%, AnalaR NORMAPUR® Reag. Ph. Eur., ACS analytical reagent
Supplier: VWR Chemicals
Urea 99.5-100.5%, AnalaR NORMAPUR® Reag. Ph. Eur., ACS analytical reagent
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Urea, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce Urea is ≥99% pure, crystalline urea, a protein denaturant with low UV absorptivity. In addition to increasing solubility of hydrophobic molecules, urea is a general protein denaturant, unfolding proteins and altering their three-dimensional structures.
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Urea 40% in aqueous solution, Sigma-Aldrich®
Supplier: Merck
Urea 40% in aqueous solution, Sigma-Aldrich®
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Urea ≥99.5%, Ultrapure for molecular biology
Supplier: Thermo Fisher Scientific
Urea ≥99.5%, Ultrapure for molecular biology
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Urea ≥98%, white powder for molecular biology
Supplier: MP Biomedicals
Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C
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Urea ≥99%, white prills, Ultrapure
Supplier: MP Biomedicals
Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.