Transfection Reagents
Avantor offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, DNA and siRNA transfection, CRISPR/Cas 9 and more. While considering a suitable transfection reagent, it is important to identify the cell type and culture conditions. Specialized transfection reagents will address rare cell cultures, neurons, and primary cells are more difficult to manipulate for porous characteristics. Avantor also offers products for electroporation and the consumables needed to support the transfection workflow.
dNTP Mix (10 mM)
Supplier: Stemcell Technologies
A solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10 mM.
Expand 1 Items
ArciTect™ crRNA
Supplier: Stemcell Technologies
Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing.
Expand 3 Items
ArciTect™ tracrRNA Kit
Supplier: Stemcell Technologies
Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing.
Expand 3 Items
STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers
Supplier: Stemcell Technologies
For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing.
Expand 1 Items
ArciTect™ Human HPRT Positive Control Kit
Supplier: Stemcell Technologies
Positive control for CRISPR-Cas9 genome editing.
Expand 1 Items
ArciTect™ T7 Endonuclease I Kit
Supplier: Stemcell Technologies
For estimation of CRISPR-Cas9 genome editing efficiency.
Expand 2 Items
STEMscript™ cDNA Synthesis Kit with Random Primers
Supplier: Stemcell Technologies
For reverse transcription of RNA without poly(A) tails, before qPCR or RNA sequencing.
Expand 1 Items
ArciTect™ Cas9 Nuclease
Supplier: Stemcell Technologies
Cas9 nuclease (4 μg/μl) for the generation of double-strand breaks in CRISPR-Cas9 genome editing.
Expand 2 Items
ArciTect™ High-Fidelity DNA Polymerase Kit
Supplier: Stemcell Technologies
Polymerase, buffers, and dNTPs for high-fidelity PCR amplification.
Expand 1 Items
ArciTect™ Annealing Buffer (5X)
Supplier: Stemcell Technologies
For guide RNA generation in CRISPR-Cas9 genome editing.
Expand 1 Items
ArciTect™ Cas9-eGFP Nuclease
Supplier: Stemcell Technologies
Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing.